概述

  • 产品名称Anti-IFI27抗体
    参阅全部 IFI27 一抗
  • 描述
    兔多克隆抗体to IFI27
  • 经测试应用适用于: ELISA, WB, IHC-Fr, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide: multiple antigenic peptide (8 branch) with the sequence

    EASALTSSAVTS

    , corresponding to amino acids 2-13 of Human IFI27

  • 阳性对照
    • SHSY5Y neuroblastoma cells

性能

应用

Our Abpromise guarantee covers the use of ab14695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000 - 1/100000.
WB Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Promotes cell death. Mediates IFN-induced apoptosis characterized by a rapid and robust release of cytochrome C from the mitochondria and activation of BAX and caspases 2, 3, 6, 8 and 9.
  • 序列相似性Belongs to the IFI6/IFI27 family.
  • 细胞定位Mitochondrion. Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2310061N23Rik antibody
    • FAM 14D antibody
    • FAM14D antibody
    • IFI 27 antibody
    • IFI27 antibody
    • IFI27_HUMAN antibody
    • Interferon alpha induced 11.5 kDa protein antibody
    • Interferon alpha inducible protein 27 antibody
    • Interferon alpha-induced 11.5 kDa protein antibody
    • Interferon alpha-inducible protein 27 antibody
    • interferon alpha-inducible protein 27, mitochondrial antibody
    • Interferon inducible protein 27 antibody
    • interferon-stimulated gene 12 antibody
    • Interferon-stimulated gene 12a protein antibody
    • ISG 12 antibody
    • ISG12 antibody
    • ISG12(a) antibody
    • ISG12(a) protein antibody
    • ISG12A antibody
    • mitochondrial antibody
    • p27 antibody
    see all

Anti-IFI27 antibody 图像

  • ICC/IF image of ab14695 stained SKNSH cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14695, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-IFI27 antibody (ab14695)参考文献

This product has been referenced in:
  • Li S  et al. Interferon alpha-inducible protein 27 promotes epithelial-mesenchymal transition and induces ovarian tumorigenicity and stemness. J Surg Res 193:255-64 (2015). Read more (PubMed: 25103640) »
  • Yang D  et al. MiR-942 Mediates Hepatitis C Virus-Induced Apoptosis via Regulation of ISG12a. PLoS One 9:e94501 (2014). WB ; Human . Read more (PubMed: 24727952) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Chimpanzee Tissue sections (Lymph Node)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization No
Specification Lymph Node
Fixative Formaldehyde
Username

Dr. Edward Greenwood

Verified customer

提交于 Jan 25 2016

resolved with credit note

Application Western blot
Sample Human Cell lysate - whole cell (293T)
Specification 293T
Gel Running Conditions Reduced Denaturing
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 04 2007

Thank you for your enquiry. Ab14695 was raised against a multiple antigenic peptide (8 branch) with the following sequence: N - EASALTSSAVTS - 'C I have updated the datasheet to reflect this information for future reference. I hope this infor...

Read More

Thank you for your enquiry and your patience in awaiting a response. The antibody was tested on thin frozen tissue sections as well as several cell lines including SHSY5Y neuroblastoma cells. If you have any further questions then please do not hesitat...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"