antigen sequence corresponding to amino acids 330-434 of Human IFFO2.
- Human duodenum tissue; RT-4 and U-251 MG cell lysates; Human Liver and Tonsil lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
Information by UniProt
- IFFO2 antibody
- IFFO2_HUMAN antibody
- Intermediate filament family orphan 2 antibody
Immunocytochemistry/ Immunofluorescence - Anti-IFFO2 antibody (ab121494)
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus and cytoplasm. Recommended concentration of ab121494 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFFO2 antibody (ab121494)
ab121494, at 1/25 dilution, staining IFFO2 in paraffin-embedded Human duodenum tissue by Immunohistochemistry.
Western blot - Anti-IFFO2 antibody (ab121494)
All lanes : Anti-IFFO2 antibody (ab121494) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"