概述

  • 产品名称Anti-IF-2(Mt)抗体
    参阅全部 IF-2(Mt) 一抗
  • 描述
    兔多克隆抗体to IF-2(Mt)
  • 经测试应用适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment corresponding to amino acids 497-646 of Human IF-2(Mt)

  • 阳性对照
    • Human cell line A431, Human plasma lysate, Human nasopharynx tissue.

性能

应用

Our Abpromise guarantee covers the use of ab121243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following is recommended: cell fixation with ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permealization with 0.1% Triton X-100 in PBS for 3x5 minutes.
WB 1/250 - 1/500.

靶标

  • 功能One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.
  • 组织特异性Expressed in all tissues examined. Highest level in skeletal muscle.
  • 序列相似性Belongs to the IF-2 family.
  • 细胞定位Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • IF-2(Mt) antibody
    • IF-2Mt antibody
    • IF2(mt) antibody
    • IF2M_HUMAN antibody
    • mitochondrial antibody
    • mitochondrial translational initiation factor 2 antibody
    • Mtif2 antibody
    • Translation initiation factor IF-2 antibody
    • Translation initiation factor IF-2, mitochondrial antibody
    see all

Anti-IF-2(Mt) antibody 图像

  • All lanes : Anti-IF-2(Mt) antibody (ab121243) at 1/250 dilution

    Lane 1 : Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
    Lane 2 : RT-4 cell lysate
    Lane 3 : U-251 MG cell lysate
    Lane 4 : Human Plasma lysate
    Lane 5 : Human liver lysate
    Lane 6 : Human tonsil lysate
  • Immunohistochemical staining of paraffin embedded Human nasopharynx tissue, using 1/25 ab121243, shows strong membranous positivity in respiratory epithelial cells.
  • Immunofluorescent staining, using 4 µg/ml ab121243, in Human cell line A431 shows positivity in mitochondria. Antibody staining is shown in green.

Anti-IF-2(Mt) antibody (ab121243)参考文献

ab121243 has not yet been referenced specifically in any publications.

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