概述

  • 产品名称
    Anti-IER5抗体
  • 描述
    山羊多克隆抗体to IER5
  • 经测试应用
    适用于: WBmore details
  • 种属反应性

    预测可用于: Human
  • 免疫原

    Synthetic peptide:

    C-QPPSGGEDDDAEE

    , corresponding to internal sequence amino acids 248-260 of Human IER5

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab59133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 34 kDa.

靶标

  • 相关性
    IER5 is a similar protein to other immediate early response proteins. In mouse, a similar protein may play an important role in mediating the cellular response to mitogenic signals.
  • 数据库链接
  • 别名
    • Immediate early response 5 antibody
    • MGC102760 antibody
    • SBBI48 antibody

图片

  • All lanes : Anti-IER5 antibody (ab59133)

    Lane 1 : Human H1299 cell lysate transfected with empty vector
    Lane 2 : Human H1299 cells lysate overexpressing IER5

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP conjugated rabbit polyclonal to goat IgG at 1/1000 dilution

    Predicted band size : 34 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an anonymous Abreview

    See Abreview

实验方案

文献

ab59133 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Sample
Human Cell lysate - whole cell (H1299 cells overexpressing IER5)
Loading amount
10 µg
Specification
H1299 cells overexpressing IER5
Gel Running Conditions
Reduced Denaturing (10 %)
Blocking step
Blocking One (Nakalai) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

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提交于 Jul 10 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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