, corresponding to internal sequence amino acids 248-260 of Human IER5
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3
Concentration information loading...
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 34 kDa.
IER5 is a similar protein to other immediate early response proteins. In mouse, a similar protein may play an important role in mediating the cellular response to mitogenic signals.
Immediate early response 5 antibody
Western blot - Anti-IER5 antibody (ab59133) This image is courtesy of an anonymous Abreview
All lanes :
Anti-IER5 antibody (ab59133)
Lane 1 :
Human H1299 cell lysate transfected with empty vector
Lane 2 :
Human H1299 cells lysate overexpressing IER5
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
HRP conjugated rabbit polyclonal to goat IgG at 1/1000 dilution
Predicted band size:
Observed band size:
45 kDa (
why is the actual band size different from the predicted?
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