Anti-IDH2抗体(ab177512)
Key features and details
- Rabbit polyclonal to IDH2
- Suitable for: IP, WB, ICC, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-IDH2抗体
参阅全部 IDH2 一抗 -
描述
兔多克隆抗体to IDH2 -
宿主
Rabbit -
经测试应用
适用于: IP, WB, ICC, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan -
免疫原
Recombinant fragment within Human IDH2 aa 402-452. The exact sequence is proprietary. NP_002159.2
Sequence:CVETVESGAMTKDLAGCIHGLSNVKLNEHFLNTTDFLDTIKSNLDRALGR Q
Database link: P48735 -
阳性对照
- WB: Whole cell lysate from 293T, HeLa, Jurkat, mouse TCMK-1 and NIH3T3 cells. IHC: Mouse renal cell carcinoma. ICC: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 - 8 -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab177512于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at 2-10 µg/mg of lysate.
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WB |
1/1000 - 1/5000. Predicted molecular weight: 51 kDa.
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ICC |
Use a concentration of 1 µg/ml.
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IHC-P |
Use a concentration of 1 µg/ml.
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说明 |
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IP
Use at 2-10 µg/mg of lysate. |
WB
1/1000 - 1/5000. Predicted molecular weight: 51 kDa. |
ICC
Use a concentration of 1 µg/ml. |
IHC-P
Use a concentration of 1 µg/ml. |
靶标
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功能
Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex. -
疾病相关
D-2-hydroxyglutaric aciduria 2
Glioma
enetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. -
序列相似性
Belongs to the isocitrate and isopropylmalate dehydrogenases family. -
翻译后修饰
Acetylation at Lys-413 dramatically reduces catalytic activity. Deacetylated by SIRT3. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 3418 Human
- Entrez Gene: 269951 Mouse
- Entrez Gene: 397603 Pig
- Omim: 147650 Human
- SwissProt: P48735 Human
- SwissProt: P54071 Mouse
- SwissProt: P33198 Pig
- Unigene: 596461 Human
see all -
别名
- D2HGA2 antibody
- ICD-M antibody
- IDH antibody
see all
图片
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Immunocytochemistry analysis of HeLa cells labelling IDH2 with ab177512 at 1 µg/mL. Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated was used as a secondary antivody at a 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal cell carcinoma tissue labelling IDH2 with ab177512 at 1 µg/mL.
Detection: DAB
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All lanes : Anti-IDH2 antibody (ab177512) at 0.4 µg/ml
Lane 1 : Whole cell lysate from 293T cells
Lane 2 : Whole cell lysate from HeLa cells
Lane 3 : Whole cell lysate from Jurkat cells
Lane 4 : Whole cell lysate from TCMK-1 cells
Lane 5 : Whole cell lysate from NIH3T3 cells
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 51 kDa
Exposure time: 30 seconds -
Lane 1: ab177512 at 1µg/ml labeling IDH2 in 293T whole cell lysate immunoprecipitated using ab177512 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 10 seconds..
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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