概述

  • 产品名称
  • 描述
    兔多克隆抗体to ID4
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Dog, Pig
  • 免疫原

    A reigon within synthetic peptide: MKAVSPVRPS GRKAPSGCGG GELALRCLAE HGHSLGGSAA AAAAAAAARC, corresponding to N terminal amino acids 1-50 of Human ID4

  • 阳性对照
    • HepG2 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab49261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 18 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration. PubMed: 21297112

靶标

图片

  • Anti-ID4 antibody (ab49261) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG 1/50000-1/100000

    Predicted band size : 18 kDa
    Observed band size : 18 kDa

文献

This product has been referenced in:
  • Jeon HM  et al. Crosstalk between glioma-initiating cells and endothelial cells drives tumor progression. Cancer Res 74:4482-92 (2014). IF . Read more (PubMed: 24962027) »
  • Phi JH  et al. ID3 contributes to cerebrospinal fluid seeding and poor prognosis in medulloblastoma. BMC Cancer 13:291 (2013). WB ; Human . Read more (PubMed: 23768125) »

See all 3 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis, postnatal day 6)
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Specification
Testis, postnatal day 6
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Mr. Bryan Niedenberger

Verified customer

提交于 Sep 15 2016

Abreviews
Application
Western blot
Sample
Rat Tissue lysate - other (spinal cord)
Loading amount
10 µg
Specification
spinal cord
Gel Running Conditions
Reduced Denaturing (10%gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Ms. Minjung Kwon

Verified customer

提交于 Jan 21 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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