ID3 overexpression 293T裂解液(全细胞) (ab94230)

概述

  • 产品名称ID3 overexpression 293T裂解液(全细胞)
  • 描述
    ID3 overexpression裂解液
  • 常规说明ab94230 is a 293T cell transfected lysate in which Human ID3 has been transiently over-expressed using a pCMV-ID3 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 背景Function: ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-3 inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. May inhibit other transcription factors. Tissue specificity: Expressed abundantly in lung, kidney and adrenal gland, but not in adult brain. Similarity: Contains 1 basic helix-loop-helix (bHLH) domain.

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应用

Our Abpromise guarantee covers the use of ab94230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

ID3 overexpression 293T lysate (whole cell) 图像

  • ab94230 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-ID3 antibody (ab55269) at 1/500 dilution

    Lane 1 : ID3 overexpression 293T lysate (whole cell) (ab94230)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

ID3 overexpression 293T lysate (whole cell) (ab94230)参考文献

ab94230 has not yet been referenced specifically in any publications.

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