Anti-ID3抗体(ab86017)
Key features and details
- Rabbit polyclonal to ID3
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
概述
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产品名称
Anti-ID3抗体
参阅全部 ID3 一抗 -
描述
兔多克隆抗体to ID3 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Recombinant fragment -
免疫原
3 synthetic peptides (Human) conjugated to KLH
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阳性对照
- Recombinant protein produced in an induced culture of E.coli.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.01% Thimerosal (merthiolate)
Constituent: Whole serum -
Concentration information loading...
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纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab86017于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Predicted molecular weight: 13 kDa.
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说明 |
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WB
1/1000. Predicted molecular weight: 13 kDa. |
靶标
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功能
ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-3 inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. May inhibit other transcription factors. -
组织特异性
Expressed abundantly in lung, kidney and adrenal gland, but not in adult brain. -
序列相似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
细胞定位
Nucleus. - Information by UniProt
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别名
- 1R21 antibody
- bHLHb25 antibody
- Class B basic helix-loop-helix protein 25 antibody
see all
图片
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All lanes : Anti-ID3 antibody (ab86017) at 1/1000 dilution
Lane 1 : Uninduced (negative control) culture of E.coli
Lane 2 : Induced culture of E.coli
Secondary
All lanes : Anti-Rabbit IgG HRP conjugated at 1/1000 dilution
Predicted band size: 13 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Additional bands at: 124 kDa, 209 kDa, 32 kDa, 45 kDa, 75 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 10%Predicted molecular weight of recombinant ID3:27kDa (13kDa + another 14kDa for the tag).
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab86017 被引用在 1 文献中.
- Chen FF et al. miR-212-5p exerts tumor promoter function by regulating the Id3/PI3K/Akt axis in lung adenocarcinoma cells. J Cell Physiol 235:7273-7282 (2020). PubMed: 32039486