概述

  • 产品名称Anti-ID3抗体
    参阅全部 ID3 一抗
  • 描述
    兔多克隆抗体to ID3
  • 经测试应用适用于: IHC-Fr, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human ID3 (C terminal).

  • 阳性对照
    • Human breast carcinoma.

性能

应用

Our Abpromise guarantee covers the use of ab41834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 20460439
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-3 inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. May inhibit other transcription factors.
  • 组织特异性Expressed abundantly in lung, kidney and adrenal gland, but not in adult brain.
  • 序列相似性Contains 1 basic helix-loop-helix (bHLH) domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1R21 antibody
    • bHLHb25 antibody
    • Class B basic helix-loop-helix protein 25 antibody
    • DNA binding protein inhibitor ID 3 antibody
    • DNA-binding protein inhibitor ID-3 antibody
    • HEIR 1 antibody
    • HEIR1 antibody
    • Helix loop helix protein HEIR 1 antibody
    • Helix loop helix protein HEIR1 antibody
    • Helix-loop-helix protein HEIR-1 antibody
    • HLH642 antibody
    • ID 3 antibody
    • ID like protein inhibitor HLH 1R21 antibody
    • ID like protein inhibitor HLH 462 antibody
    • ID-like protein inhibitor HLH 1R21 antibody
    • ID3 antibody
    • ID3_HUMAN antibody
    • Inhibitor of DNA binding 3 antibody
    • Inhibitor of DNA binding 3 dominant negative helix loop helix protein antibody
    see all

Anti-ID3 antibody 图像

  • Ab41834 staining human ID3 in human breast carcinoma by immunohistochemistry using formalin fixed, paraffin embedded tissue.
  • ICC/IF image of ab41834 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41834, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-ID3 antibody (ab41834)参考文献

This product has been referenced in:
  • Forni PE  et al. The indirect role of fibroblast growth factor-8 in defining neurogenic niches of the olfactory/GnRH systems. J Neurosci 33:19620-34 (2013). Mouse . Read more (PubMed: 24336726) »
  • Gajera CR  et al. LRP2 in ependymal cells regulates BMP signaling in the adult neurogenic niche. J Cell Sci 123:1922-30 (2010). IHC-Fr ; Mouse . Read more (PubMed: 20460439) »

See all 2 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (breast cancer, prostate cancer, kidney)
Specification breast cancer, prostate cancer, kidney
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate, 20 minutes at 100C
Permeabilization No
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提交于 Feb 17 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"