概述

  • 产品名称Anti-ID3抗体[2B11]
    参阅全部 ID3 一抗
  • 描述
    小鼠单克隆抗体[2B11] to ID3
  • 经测试应用适用于: IP, WB, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant full length protein (Human) ID3 expressed in E. coli.

性能

应用

Our Abpromise guarantee covers the use of ab36501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 16 kDa.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-3 inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. May inhibit other transcription factors.
  • 组织特异性Expressed abundantly in lung, kidney and adrenal gland, but not in adult brain.
  • 序列相似性Contains 1 basic helix-loop-helix (bHLH) domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1R21 antibody
    • bHLHb25 antibody
    • Class B basic helix-loop-helix protein 25 antibody
    • DNA binding protein inhibitor ID 3 antibody
    • DNA-binding protein inhibitor ID-3 antibody
    • HEIR 1 antibody
    • HEIR1 antibody
    • Helix loop helix protein HEIR 1 antibody
    • Helix loop helix protein HEIR1 antibody
    • Helix-loop-helix protein HEIR-1 antibody
    • HLH642 antibody
    • ID 3 antibody
    • ID like protein inhibitor HLH 1R21 antibody
    • ID like protein inhibitor HLH 462 antibody
    • ID-like protein inhibitor HLH 1R21 antibody
    • ID3 antibody
    • ID3_HUMAN antibody
    • Inhibitor of DNA binding 3 antibody
    • Inhibitor of DNA binding 3 dominant negative helix loop helix protein antibody
    see all

Anti-ID3 antibody [2B11] 图像

  • Overlay histogram showing HeLa cells stained with ab36501 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab36501, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-ID3 antibody [2B11] (ab36501)参考文献

This product has been referenced in:
  • Lee D  et al. Id proteins regulate capillary repair and perivascular cell proliferation following ischemia-reperfusion injury. PLoS One 9:e88417 (2014). WB ; Mouse . Read more (PubMed: 24516656) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Zebrafish Tissue sections (Retina, Muller cell)
Permeabilization Yes - 0.1% TritonX
Specification Retina, Muller cell
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative Paraformaldehyde
Username

Dr. Ryan MacDonald

Verified customer

提交于 Nov 08 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"