Anti-ID3抗体[10D3] (ab118032)

概述

  • 产品名称Anti-ID3抗体[10D3]
    参阅全部 ID3 一抗
  • 描述
    小鼠单克隆抗体[10D3] to ID3
  • 经测试应用适用于: IHC-Fr, WB, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Human, Zebrafish
  • 免疫原

    Recombinant full length Human ID3 produced in HEK293T cells (NP_002158).

  • 阳性对照
    • HEK293T cell lysate transfected with pCMV6-ENTRY ID3 cDNA; Human liver tissue; HeLa and Jurkat cells.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab118032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/100.
WB 1/500. Predicted molecular weight: 13 kDa.
IHC-P 1/50.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-3 inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. May inhibit other transcription factors.
  • 组织特异性Expressed abundantly in lung, kidney and adrenal gland, but not in adult brain.
  • 序列相似性Contains 1 basic helix-loop-helix (bHLH) domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1R21 antibody
    • bHLHb25 antibody
    • Class B basic helix-loop-helix protein 25 antibody
    • DNA binding protein inhibitor ID 3 antibody
    • DNA-binding protein inhibitor ID-3 antibody
    • HEIR 1 antibody
    • HEIR1 antibody
    • Helix loop helix protein HEIR 1 antibody
    • Helix loop helix protein HEIR1 antibody
    • Helix-loop-helix protein HEIR-1 antibody
    • HLH642 antibody
    • ID 3 antibody
    • ID like protein inhibitor HLH 1R21 antibody
    • ID like protein inhibitor HLH 462 antibody
    • ID-like protein inhibitor HLH 1R21 antibody
    • ID3 antibody
    • ID3_HUMAN antibody
    • Inhibitor of DNA binding 3 antibody
    • Inhibitor of DNA binding 3 dominant negative helix loop helix protein antibody
    see all

Anti-ID3 antibody [10D3] 图像

  • ab118032 staining ID3 in zebrafish retina sections by IHC-Fr. The tissue was fixed with paraformaldehyde and an antigen retrieval step was performed with sodium citrate pH 6. Blocking of the sample was done with 5% BSA in PBS containing 01% Tween 20 and 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab118032 at 1/100 in blocking solution for 16h at 4°C. An alexa 647 conjugated goat anti-mouset polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. ID3 expression can be observed in Muller cells.

    See Abreview

  • All lanes : Anti-ID3 antibody [10D3] (ab118032) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY ID3 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 13 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200583)
  • ab118032 at 1/50 dilution staining ID3 in Paraffin-embedded Human Liver tissue by Immunohistochemistry.
  • ab118032 at 1/100 dilution staining ID3 in HeLa cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
  • ab118032 at 1/100 dilution staining ID3 in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).

Anti-ID3 antibody [10D3] (ab118032)参考文献

ab118032 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample Zebrafish Tissue sections (Retina, Muller Cell)
Specification Retina, Muller Cell
Permeabilization Yes - 0.1% Triton X
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 07 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

Read More

Thank you for taking the time to contact and update me. I am sorry to hear the suggestions and protocol have not improved the results on this occasion.

I appreciate the time you have spent in the laboratory and understand your concerns. It i...

Read More

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this...

Read More

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"