ID2 overexpression 293T裂解液(全细胞) (ab94214)

概述

  • 产品名称ID2 overexpression 293T裂解液(全细胞)
  • 描述
    ID2 overexpression裂解液
  • 常规说明ab94214 is a 293T cell transfected lysate in which Human ID2 has been transiently over-expressed using a pCMV-ID2 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 背景Developmental stage: Found in most early fetal tissues but not in the corresponding mature tissues. Function: ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression. Similarity: Contains 1 basic helix-loop-helix (bHLH) domain. Tissue specificity: Highly expressed in early fetal tissues, including those of the central nervous system.

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应用

Our Abpromise guarantee covers the use of ab94214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

ID2 overexpression 293T lysate (whole cell) 图像

  • ab94214 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-ID2 antibody (ab89530) at 1/500 dilution

    Lane 1 : ID2 overexpression 293T lysate (whole cell) (ab94214)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugate at 1/2500 dilution

ID2 overexpression 293T lysate (whole cell) (ab94214)参考文献

ab94214 has not yet been referenced specifically in any publications.

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