概述

  • 产品名称Anti-ID2抗体
    参阅全部 ID2 一抗
  • 描述
    小鼠单克隆抗体to ID2
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length human ID2 protein (AAH30639) with a 26 kDa tag

  • 阳性对照
    • HeLa cell lysate, HepG2 cell lysate and ID2 transfected 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab89530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 15 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression.
  • 组织特异性Highly expressed in early fetal tissues, including those of the central nervous system.
  • 序列相似性Contains 1 basic helix-loop-helix (bHLH) domain.
  • 发展阶段Found in most early fetal tissues but not in the corresponding mature tissues.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bHLHb26 antibody
    • Cell growth inhibiting gene 8 antibody
    • class B basic helix loop helix protein 26 antibody
    • Class B basic helix-loop-helix protein 26 antibody
    • DNA binding protein inhibitor ID 2 antibody
    • DNA binding protein inhibitor ID2 antibody
    • DNA-binding protein inhibitor ID-2 antibody
    • GIG 8 antibody
    • GIG8 antibody
    • Helix loop helix protein ID2 antibody
    • ID2 antibody
    • ID2_HUMAN antibody
    • ID2A antibody
    • ID2H antibody
    • Inhibitor of differentiation 2 antibody
    • Inhibitor of DNA binding 2 antibody
    • Inhibitor of DNA binding 2, dominant negative helix loop helix protein antibody
    • MGC26389 antibody
    see all

Anti-ID2 antibody 图像

  • Anti-ID2 antibody (ab89530) at 5 µg/ml + recombinant protein (the immunogen) at 0.2 µg

    Predicted band size : 15 kDa
    Observed band size : 41 kDa (why is the actual band size different from the predicted?)
  • Anti-ID2 antibody (ab89530) at 5 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 15 kDa
    Observed band size : 15 kDa
  • Anti-ID2 antibody (ab89530) at 5 µg/ml + HepG2 cell lysate at 50 µg

    Predicted band size : 15 kDa
    Observed band size : 15 kDa
  • All lanes : Anti-ID2 antibody (ab89530) at 5 µg/ml

    Lane 1 : ID2 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 15 kDa
    Observed band size : 15 kDa

Anti-ID2 antibody (ab89530)参考文献

ab89530 has not yet been referenced specifically in any publications.

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