概述

  • 产品名称
  • 描述
    兔多克隆抗体to ID2
  • 经测试应用
    适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    3 synthetic peptides (Human) conjugated to KLH

  • 阳性对照
    • WB: Recombinant protein produced in an induced culture of E.coli. ICC/IF: NIH3T3 cells. IHC-P: Human breast adenocarcinoma tissue.

性能

应用

Our Abpromise guarantee covers the use of ab85990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200.
IHC-P 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 15 kDa).

靶标

  • 功能
    ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression.
  • 组织特异性
    Highly expressed in early fetal tissues, including those of the central nervous system.
  • 序列相似性
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • 发展阶段
    Found in most early fetal tissues but not in the corresponding mature tissues.
  • 细胞定位
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bHLHb26 antibody
    • Cell growth inhibiting gene 8 antibody
    • class B basic helix loop helix protein 26 antibody
    • Class B basic helix-loop-helix protein 26 antibody
    • DNA binding protein inhibitor ID 2 antibody
    • DNA binding protein inhibitor ID2 antibody
    • DNA-binding protein inhibitor ID-2 antibody
    • GIG 8 antibody
    • GIG8 antibody
    • Helix loop helix protein ID2 antibody
    • ID2 antibody
    • ID2_HUMAN antibody
    • ID2A antibody
    • ID2H antibody
    • Inhibitor of differentiation 2 antibody
    • Inhibitor of DNA binding 2 antibody
    • Inhibitor of DNA binding 2, dominant negative helix loop helix protein antibody
    • MGC26389 antibody
    see all

图片

  • IHC image of ID2 staining in Human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85990, 1/1000, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-ID2 antibody (ab85990) at 1/1000 dilution

    Lane 1 : Uninduced (negative control) culture of E.coli
    Lane 2 : Induced culture of E.coli

    Secondary
    anti-Rabbit IgG HRP conjugated at 1/1000 dilution

    Predicted band size : 15 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
    Predicted molecular weight of Recombinant ID2:29 kDa (15 kDa + another 14 kDa for the tag).
  • ICC/IF image of ab85990 stained NIH-3T3 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85990, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Ladle BH  et al. De novo DNA methylation by DNA methyltransferase 3a controls early effector CD8+ T-cell fate decisions following activation. Proc Natl Acad Sci U S A 113:10631-6 (2016). Flow Cyt ; Mouse . Read more (PubMed: 27582468) »

See 1 Publication for this product

客户评价及客户问答

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Human embryonic lung - 7 weeks post conception)
Permeabilization
Yes - 10 min 0.3% triton post-sectioning
Specification
Human embryonic lung - 7 weeks post conception
Blocking step
1% BSA, 5% normal donkey serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Ms. Emma Rawlins

Verified customer

提交于 Oct 27 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (NSCs neural stem cells)
Permeabilization
No
Specification
NSCs neural stem cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 26 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Embryonic Heart)
Permeabilization
Yes - Tween-20
Specification
Embryonic Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 27 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
30 µg
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 20 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Embryonic pancreas)
Permeabilization
Yes - 1% Triton
Specification
Embryonic pancreas
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 29 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册