概述

  • 产品名称
  • 描述
    兔多克隆抗体to ID2
  • 经测试应用
    适用于: ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A synthetic peptide from C-terminal of human ID2 protein

  • 阳性对照
    • Breast carcinoma.

性能

应用

Our Abpromise guarantee covers the use of ab52093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
IHC-P
  • 应用说明
    IHC-P: 1/50 for 30 min at RT.
    Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression.
    • 组织特异性
      Highly expressed in early fetal tissues, including those of the central nervous system.
    • 序列相似性
      Contains 1 basic helix-loop-helix (bHLH) domain.
    • 发展阶段
      Found in most early fetal tissues but not in the corresponding mature tissues.
    • 细胞定位
      Cytoplasm. Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • bHLHb26 antibody
      • Cell growth inhibiting gene 8 antibody
      • class B basic helix loop helix protein 26 antibody
      • Class B basic helix-loop-helix protein 26 antibody
      • DNA binding protein inhibitor ID 2 antibody
      • DNA binding protein inhibitor ID2 antibody
      • DNA-binding protein inhibitor ID-2 antibody
      • GIG 8 antibody
      • GIG8 antibody
      • Helix loop helix protein ID2 antibody
      • ID2 antibody
      • ID2_HUMAN antibody
      • ID2A antibody
      • ID2H antibody
      • Inhibitor of differentiation 2 antibody
      • Inhibitor of DNA binding 2 antibody
      • Inhibitor of DNA binding 2, dominant negative helix loop helix protein antibody
      • MGC26389 antibody
      see all

    图片

    • Staining of ID2 in human breast carcinoma by immunohistochemistry, using ab52093 (1/50). (Formalin fixed, Paraffin embedded section).

    文献

    This product has been referenced in:

    See all 2 Publications for this product

    客户评价及客户问答

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    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Human embryonic lung - 7 weeks post conception)
    Permeabilization
    Yes - 10 min 0.3% triton post-sectioning
    Specification
    Human embryonic lung - 7 weeks post conception
    Blocking step
    1% BSA, 5% normal donkey serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Username

    Ms. Emma Rawlins

    Verified customer

    提交于 May 24 2017

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Sample
    Mouse Cell (bEnd.3)
    Specification
    bEnd.3
    Permeabilization
    Yes - 0.1% Triton X100
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Jul 14 2014

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Sample
    Human Cell (HUVEC)
    Specification
    HUVEC
    Permeabilization
    Yes - 0.1% Triton X100
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Jul 02 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (breast cancer, prostate cancer)
    Specification
    breast cancer, prostate cancer
    Fixative
    Formaldehyde
    Antigen retrieval step
    None
    Permeabilization
    No
    Username

    Abcam user community

    Verified customer

    提交于 Feb 09 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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