ab53545 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Detects a band of approximately 32 kDa (predicted molecular weight: 15 kDa).
Use at an assay dependent dilution.
ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression.
Highly expressed in early fetal tissues, including those of the central nervous system.
Contains 1 basic helix-loop-helix (bHLH) domain.
Found in most early fetal tissues but not in the corresponding mature tissues.