概述

  • 产品名称Anti-ICAM3抗体[ICAM3.1]
    参阅全部 ICAM3 一抗
  • 描述
    小鼠单克隆抗体[ICAM3.1] to ICAM3
  • 经测试应用适用于: WB, IP, ELISA, RIA, IHC-Fr, ICC/IF, Flow Cyt, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    ICAM 3Fc chimeric fusion protein.

性能

应用

Our Abpromise guarantee covers the use of ab10804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
ELISA
RIA
IHC-Fr
ICC/IF
Flow Cyt
IHC-P
  • 应用说明ELISA: Use at an assay dependent dilution.
    Flow Cyt: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 1 µg/ml.
    IP: Use at an assay dependent dilution.
    RIA: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 66 kDa.
    ICC/IF: Use at an assay dependent dilution From (PubMed:17353362)
    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2.
    • 序列相似性Belongs to the immunoglobulin superfamily. ICAM family.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    • 翻译后修饰Upon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues.
      N-glycosylated; glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CD50 antibody
      • CDW 50 antibody
      • CDw50 antibody
      • ICAM 3 antibody
      • ICAM-3 antibody
      • ICAM-R antibody
      • ICAM3 antibody
      • ICAM3_HUMAN antibody
      • ICAMR antibody
      • Intercellular adhesion molecule 3 antibody
      see all

    Anti-ICAM3 antibody [ICAM3.1] 图像

    • Ab10804 staining Human malignant stomach. Staining is localised to the membrane.
      Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
      Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
    • ab10804 staining ICAM3 in Y27632-treated CEM T cells by Immunocytochemistry/ Immunofluorescence.
      CEM T cells were fixed for 15 minutes with 4% paraformaldehyde. Cells were permeabilized with 0.1% Triton X-100, blocked with 1% BSA, and incubated with ab10804 (1/50), for 1 hour followed by Alexa Fluor 488–conjugated anti–mouse IgG secondary antibodies (1/200).

    Anti-ICAM3 antibody [ICAM3.1] (ab10804)参考文献

    This product has been referenced in:
    • Mintz PJ  et al. Exploiting human CD34+ stem cell-conditioned medium for tissue repair. Mol Ther 22:149-59 (2014). Read more (PubMed: 23985698) »
    • Heasman SJ  et al. Coordinated RhoA signaling at the leading edge and uropod is required for T cell transendothelial migration. J Cell Biol 190:553-63 (2010). ICC/IF ; Human . Read more (PubMed: 20733052) »

    See all 3 Publications for this product

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