RabMAb

Anti-ICAM3抗体[EPR3994] (ab109405)

概述

  • 产品名称Anti-ICAM3抗体[EPR3994]
    参阅全部 ICAM3 一抗
  • 描述
    兔单克隆抗体[EPR3994] to ICAM3
  • 经测试应用适用于: ICC, WB, IP, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues in the extracellular domain of Human ICAM3.

  • 阳性对照
    • U937, Jurkat, HuT-78, Ramos, and THP-1 cell lysates; Human spleen, colon and tonsil tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab109405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC 1/100 - 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IP 1/10 - 1/100.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2.
  • 序列相似性Belongs to the immunoglobulin superfamily. ICAM family.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • 翻译后修饰Upon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues.
    N-glycosylated; glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD50 antibody
    • CDW 50 antibody
    • CDw50 antibody
    • ICAM 3 antibody
    • ICAM-3 antibody
    • ICAM-R antibody
    • ICAM3 antibody
    • ICAM3_HUMAN antibody
    • ICAMR antibody
    • Intercellular adhesion molecule 3 antibody
    see all

Anti-ICAM3 antibody [EPR3994] 图像

  • All lanes : Anti-ICAM3 antibody [EPR3994] (ab109405) at 1/1000 dilution

    Lane 1 : U937 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HuT-78 cell lysate
    Lane 4 : Ramos cell lysate
    Lane 5 : THP-1 cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 60 kDa
  • Immunohistochemical analysis of ICAM3 in paraffin-embedded Human spleen tissue using ab109405 at 1/500 dilution.
  • Immunohistochemical analysis of ICAM3 in paraffin-embedded Human colon tissue using ab109405 at 1/500 dilution.
  • Immunohistochemical analysis of ICAM3 in paraffin-embedded Human tonsil tissue using ab109405 at 1/500dilution.

Anti-ICAM3 antibody [EPR3994] (ab109405)参考文献

ab109405 has not yet been referenced specifically in any publications.

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