概述

  • 产品名称Anti-ICAM3抗体
    参阅全部 ICAM3 一抗
  • 描述
    兔多克隆抗体to ICAM3
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human ICAM3.

    (Peptide available as ab90838.)

  • 阳性对照
    • This antibody gave a positive signal in human spleen tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab78097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
IHC-P Use a concentration of 5 µg/ml.

靶标

  • 功能ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2.
  • 序列相似性Belongs to the immunoglobulin superfamily. ICAM family.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • 翻译后修饰Upon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues.
    N-glycosylated; glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD50 antibody
    • CDW 50 antibody
    • CDw50 antibody
    • ICAM 3 antibody
    • ICAM-3 antibody
    • ICAM-R antibody
    • ICAM3 antibody
    • ICAM3_HUMAN antibody
    • ICAMR antibody
    • Intercellular adhesion molecule 3 antibody
    see all

Anti-ICAM3 antibody 图像

  • Anti-ICAM3 antibody (ab78097) at 1 µg/ml + Human spleen tissue lysate - total protein (ab29699) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 59 kDa
    Observed band size : 59 kDa
    Additional bands at : 34 kDa,36 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • IHC image of ab78097 staining in Hodgekin's Lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78097, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-ICAM3 antibody (ab78097)参考文献

ab78097 has not yet been referenced specifically in any publications.

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