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Our Abpromise guarantee covers the use of ab25375 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration. PubMed: 2573511|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 8943409|
|Blocking||Use at an assay dependent concentration. PubMed: 2981916|
|WB||Use at an assay dependent concentration. Detects a band of approximately 95 kDa (predicted molecular weight: 85-110 kDa). PubMed: 8095510|
Flow Cytometry analysis of BALB/c mouse splenocytes labeling ICAM1 with ab25375 at 0.3 μg/106 cells (purple). A Mouse Anti-Rat IgG2b-CY5 was used as the secondary antibody. Grey - Isotype Control, Rat IgG2b-UNLB, followed by Mouse Anti-Rat IgG2b-CY5.
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