The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: 1/25 - 1/100. Use 10µl working dilution for 106 cells in 100µl.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
Belongs to the immunoglobulin superfamily. ICAM family. Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
Tamatani T et al. Molecular mechanisms underlying lymphocyte recirculation. II. Differential regulation of LFA-1 in the interaction between lymphocytes and high endothelial cells. Eur J Immunol21:855-8 (1991).
Read more (PubMed: 1672652) »
Tamatani T & Miyasaka M Identification of monoclonal antibodies reactive with the rat homolog of ICAM-1, and evidence for a differential involvement of ICAM-1 in the adherence of resting versus activated lymphocytes to high endothelial cells. Int Immunol2:165-71 (1990).
Read more (PubMed: 2088483) »