ICAD overexpression 293T裂解液(全细胞) (ab94130)

概述

  • 产品名称ICAD overexpression 293T裂解液(全细胞)
  • 描述
    ICAD overexpression裂解液
  • 常规说明ab94130 is a 293T cell transfected lysate in which Human ICAD has been transiently over-expressed using a pCMV-ICAD plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 背景Function: Inhibitor of the caspase-activated DNase (DFF40). Similarity: Contains 1 CIDE-N domain. PTM: Caspase-3 cleaves DFF45 at 2 sites to generate an active factor. Phosphorylated upon DNA damage, probably by ATM or ATR.

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应用

Our Abpromise guarantee covers the use of ab94130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

ICAD overexpression 293T lysate (whole cell) 图像

  • ab94130 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-ICAD antibody (ab90563) at 1/500 dilution

    Lane 1 : ICAD overexpression 293T lysate (whole cell) (ab94130)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

ICAD overexpression 293T lysate (whole cell) (ab94130)参考文献

ab94130 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"