Anti-IBTK抗体(ab107852)

概述

  • 产品名称Anti-IBTK抗体
  • 描述
    兔多克隆抗体to IBTK
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 589-617 of Human IBTK (NP_056340.2)

  • 阳性对照
    • MDA-MB435 cell line lysates Human lymph tissue

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody is purified through a protein A column, followed by peptide affinity purification.
  • 克隆多克隆
  • 同种型IgG

应用

Our Abpromise guarantee covers the use of ab107852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 151 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Acts as an inhibitor of BTK tyrosine kinase activity, thereby playing a role in B-cell development. Down-regulates BTK kinase activity, leading to interference with BTK-mediated calcium mobilization and NF-kappa-B-driven transcription.
  • 组织特异性Expressed in DeFew, 293T, Hela and in Jurkat, MC3 and NB4 lymphoid cells (at protein level). Isoform 1 is the predominant isoform expressed in all examined tissues and cell lines. Highly expressed in hemopoietic tissues (fetal liver, spleen, lymph node, thymus, peripheral blood leukocytes and bone marrow). Weakly or not expressed in other tissues.
  • 序列相似性Contains 3 ANK repeats.
    Contains 2 BTB (POZ) domains.
    Contains 3 RCC1 repeats.
  • 细胞定位Nucleus and Cytoplasm. Membrane. Translocates to the plasma membrane upon IgM stimulation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BTKI antibody
    • IBtk antibody
    • IBTK_HUMAN antibody
    • inhibitor of Bruton agammaglobulinemia tyrosine kinase antibody
    • Inhibitor of Bruton tyrosine kinase antibody
    see all

Anti-IBTK antibody 图像

  • Anti-IBTK antibody (ab107852) at 1/100 dilution + MDA-MB435 cell line lysates at 35 µg

    Predicted band size : 151 kDa
  • Immunohistochemistry analysis in formalin fixed and paraffin embedded Human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. ab107852 at 1/50.

Anti-IBTK antibody (ab107852)参考文献

ab107852 has not yet been referenced specifically in any publications.

Product Wall

Application Immunoprecipitation
Sample Human Cell lysate - whole cell (Defew B-cell Lymphoma)
Total protein in input 1000 µg
Immuno-precipitation step Other - TrueBlot anti-rabbit beads
Specification Defew B-cell Lymphoma
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提交于 May 28 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"