功能E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se.
组织特异性Broadly expressed, with lowest levels in brain and thymus, and highest levels detectable in heart, ovary and testis.
通路Protein modification; protein ubiquitination.
序列相似性Belongs to the RBR family. RNF144 subfamily. Contains 1 IBR-type zinc finger. Contains 2 RING-type zinc fingers.
结构域The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme (By similarity). The transmembrane domain is essential for translocation to the mitochondria upon induction of apoptosis.
细胞定位Mitochondrion membrane. Cytoplasm. Mostly cytosololic, accumulates in submitochondrial domains specifically upon apoptosis induction, in synchrony with BAX activation.