参阅全部 IBRDC1 一抗
WB, ELISAmore details
Synthetic peptide corresponding to a region within internal sequence amino acids 216-265 (GLALGAIAVV IVEEIKTYWN LISGRTRNQT QHLAPQPVLL SDMLYCLKQV) of human IBRDC1 (NP_689766).
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- 存储溶液Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
- 纯度Immunogen affinity purified
- 纯化说明ab83812 is purified by a peptide affinity chromatography method.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/1562500.
- 相关性IBRDC1 is an E3 ubiquitin protein ligase which accepts ubiquitin from E2 ubiquitin conjugating enzymes in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.
- 细胞定位Membrane; Single pass membrane protein.
- C6orf172 antibody
- dJ84N20.1 antibody
- IBR domain containing 1 antibody
Anti-IBRDC1 antibody 图像
Western blot - IBRDC1 antibody (ab83812)
Anti-IBRDC1 antibody (ab83812) at 1 µg/ml + fetal heart lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 32 kDa
Observed band size : 28 kDa (why is the actual band size different from the predicted?)
Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
Anti-IBRDC1 antibody (ab83812)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"