概述

  • 产品名称
  • 描述
    山羊多克隆抗体to Iba1
  • 特异性
    This antibody is expected to recognise isoform 3 (NP_001614.3) only. This antibody gave excellent staining in paraffin embedded human adult tissue and embyronic rat tissue. For WB, we recommend blocking in 3-5% milk, NOT BSA. Blocking in BSA gives high background.
  • 经测试应用
    适用于: WB, IHC-Fr, IHC-Pmore details
    不适用于: IHC-FoFr
  • 种属反应性
    与反应: Mouse, Rabbit, Human
    预测可用于: Cow, Pig, Macaque monkey
  • 免疫原

    Synthetic peptide corresponding to Human Iba1 aa 28-42 (internal sequence) (Cysteine residue). NP_001614.3
    Sequence:

    C-NKQFLDDPKYSSDED


    Database link: P55008

  • 阳性对照
    • WB: Human peripheral blood mononucleocyte and frontal cortex lysate. IHC-P: Mouse brain; Human placenta and jejunum; rat embryonic liver tissue.

性能

应用

Our Abpromise guarantee covers the use of ab48004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).

We recommend blocking in 3-5% milk, not BSA. Blocking in BSA gives high background in WB.

IHC-Fr Use at an assay dependent concentration.
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • 应用说明
    Is unsuitable for IHC-FoFr.
  • 靶标

    • 功能
      Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
    • 组织特异性
      Detected in T-lymphocytes and peripheral blood mononuclear cells.
    • 序列相似性
      Contains 2 EF-hand domains.
    • 翻译后修饰
      Phosphorylated on serine residues.
    • 细胞定位
      Cytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AIF 1 antibody
      • AIF-1 antibody
      • Aif1 antibody
      • AIF1 protein antibody
      • AIF1_HUMAN antibody
      • Allograft inflammatory factor 1 antibody
      • Allograft inflammatory factor 1 splice variant G antibody
      • allograft inflammatory factor-1 splice variant Hara-1 antibody
      • balloon angioplasty responsive transcription antibody
      • BART 1 antibody
      • G1 antibody
      • G1 putative splice variant of allograft inflamatory factor 1 antibody
      • IBA 1 antibody
      • IBA1 antibody
      • interferon gamma responsive transcript antibody
      • Interferon responsive transcript 1 antibody
      • interferon responsive transcript factor 1 antibody
      • Ionized calcium binding adapter molecule 1 antibody
      • Ionized calcium-binding adapter molecule 1 antibody
      • ionized calcium-binding adapter molecule antibody
      • IRT 1 antibody
      • IRT1 antibody
      • Microglia response factor antibody
      • MRF1 antibody
      • Protein g1 antibody
      see all

    图片

    • Paraffin embedded mouse brain tissue stained for Iba1 with ab48004 (2 µg/ml) in immunohistochemical analysis. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

    • Anti-Iba1 antibody (ab48004) at 0.5 µg/ml + human frontal cortex lysate in RIPA buffer at 35 µg
      Developed using the ECL technique

      Predicted band size : 17 kDa

      Primary incubation was 1 hour

    • Paraformaldehyde fixed mouse brain tissue stained for Iba1 with ab48004 (1/200 dilution) in immunohistochemical analysis. Secondary: Donkey Anti-Goat IgG Cy3® (1/500 dilution).

      See Abreview

    • All lanes : Anti-Iba1 antibody (ab48004) at 1 µg/ml

      Lane 1 : Human peripheral blood mononucleocyte lysate in RIPA buffer
      Lane 2 : Human peripheral blood mononucleocyte lysate in RIPA buffer with blocking peptide

      Lysates/proteins at 35 µg per lane.

      Developed using the ECL technique

      Predicted band size : 17 kDa
      Observed band size : 17 kDa
      Additional bands at : 110 kDa,35 kDa. We are unsure as to the identity of these extra bands.1 hour primary antibody incubation. Additional bands of unknown identity observed at 35kDa and 110kDa. These bands were successfully blocked by incubation with the immunising peptide
    • ab48004 at 1/300 staining human jejunum tissue sections by IHC-P. A heat mediated antigen retrieval step in citric acid buffer was performed and the tissue was blocked in BSA before incubation with the antibody for 2 hours. A biotinylated rabbit anti-goat antibody was used as the secondary.

      See Abreview

    • ab48004 diluted 1/200 with TBS/BSA/Azide staining rat d15 embryonic liver tissue sections by IHC-P.  The tissue was formaldehyde fixed and a heat-mediated antigen retrieval step in citric acid buffer at pH6 was performed prior to blocking with 1% BSA for 10 minutes at room temperature.  Incubation time with the primary antibody was 16 minutes.  A biotinylated rabbit anti-goat was used as the secondary.

      See Abreview

    • Paraffin embedded human placenta tissue stained for Iba1 with ab48004 (2.5 µg/ml) in immunohistochemical analysis. A heat mediated antigen retrieval step in citric acid buffer (pH 6) was performed.

    文献

    This product has been referenced in:
    • Hurtado-Alvarado G  et al. A2A Adenosine Receptor Antagonism Reverts the Blood-Brain Barrier Dysfunction Induced by Sleep Restriction. PLoS One 11:e0167236 (2016). WB, IHC ; Rat . Read more (PubMed: 27893847) »
    • Díaz Galindo C  et al. Leuprolide acetate induces structural and functional recovery of injured spinal cord in rats. Neural Regen Res 10:1819-24 (2015). IHC . Read more (PubMed: 26807118) »

    See all 2 Publications for this product

    客户评价及客户问答

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (spinal cord)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.3% TritonX-100
    Specification
    spinal cord
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Username

    Jennifer Deberry

    Verified customer

    提交于 Mar 15 2016

    Thank you for your enquiry regarding ab48004 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is having problems with this antibody.

    Though you have kindly provided some det...

    Read More

    Thank you for your reply.

    Your protocol seems comprehensive, although I would strongly recommend including the antigen retrieval step since this has worked well for other customers. We would recommend citrate buffer pH 6, which we do sell as ...

    Read More

    Thank you for contacting us.

    I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

    Wou...

    Read More
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Brain)
    Specification
    Brain
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer
    Permeabilization
    Yes - 0.1% TritonX-100
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Jul 13 2012

    Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    The details you have kindly provided will enable us to investigate this case for you and this is also ...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rabbit Tissue sections (brain)
    Specification
    brain
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - triton (20 ul in 10 ml PBS)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Praveen Ballabh

    Verified customer

    提交于 Jul 22 2010

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Dog Tissue sections (spinal cord)
    Specification
    spinal cord
    Fixative
    formalin
    Permeabilization
    Yes - 0.3% PBS-TX
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Joanne Steinauer

    Verified customer

    提交于 Jun 08 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (brain)
    Specification
    brain
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5%TritonX-100/PBS
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Jul 15 2009

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Jul 07 2009

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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