概述

  • 产品名称Anti-Iba1抗体
    参阅全部 Iba1 一抗
  • 描述
    山羊多克隆抗体to Iba1
  • 特异性For WB, Abcam recommends blocking in milk (3-5%) for cleaner blots with reduced background, in comparison to BSA. If looking for a monoclonal anti-Iba1 alternative we can recommend our RabMAb ab178846. This has the same immunogen as ab5076.
  • 经测试应用适用于: IHC-FrFl, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Dog, Human, Marmoset (common)
    预测可用于: Pig, Macaque Monkey, Chinese Hamster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Rat Iba1.

  • 阳性对照
    • Rat Cortex Tissue Lysate; Mouse spleen lysate; Rat spleen lysate; THP1 whole cell lysate; NR8383 whole cell lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab107159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FrFl Use at an assay dependent concentration. PubMed: 25068145
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).

Abcam recommends blocking in milk (3-5%) for cleaner blots with reduced background, in comparison to BSA. 

靶标

  • 功能Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
  • 组织特异性Detected in T-lymphocytes and peripheral blood mononuclear cells.
  • 序列相似性Contains 2 EF-hand domains.
  • 翻译后修饰Phosphorylated on serine residues.
  • 细胞定位Cytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AIF 1 antibody
    • AIF-1 antibody
    • Aif1 antibody
    • AIF1 protein antibody
    • AIF1_HUMAN antibody
    • Allograft inflammatory factor 1 antibody
    • Allograft inflammatory factor 1 splice variant G antibody
    • allograft inflammatory factor-1 splice variant Hara-1 antibody
    • balloon angioplasty responsive transcription antibody
    • BART 1 antibody
    • G1 antibody
    • G1 putative splice variant of allograft inflamatory factor 1 antibody
    • IBA 1 antibody
    • IBA1 antibody
    • interferon gamma responsive transcript antibody
    • Interferon responsive transcript 1 antibody
    • interferon responsive transcript factor 1 antibody
    • Ionized calcium binding adapter molecule 1 antibody
    • Ionized calcium-binding adapter molecule 1 antibody
    • ionized calcium-binding adapter molecule antibody
    • IRT 1 antibody
    • IRT1 antibody
    • Microglia response factor antibody
    • MRF1 antibody
    • Protein g1 antibody
    see all

Anti-Iba1 antibody 图像

  • Anti-Iba1 antibody (ab107159) at 1 µg/ml + Rat Cortex Tissue Lysate at 10 µg

    Secondary
    Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 17 kDa
    Observed band size : 17 kDa


    Exposure time : 20 minutes
  • IHC-P image of Iba1 staining on marmoset (common) kidney sections using ab107159 (1:5000). The image shows macrophage infiltration in the cortex. The paraffin embedded sectons were subjected to heat mediated antigen retrieval using citric acid. The sections was then blocked using 1% BSA at 21°C for 10 min. The primary antibody was incubated at 21°C for 16 hours. The secondary antibody was rabbit polyclonal to sheep IgG conjugated to biotin at 1:200.

    See Abreview

  • All lanes : Anti-Iba1 antibody (ab107159) at 2 µg/ml

    Lane 1 : Human Iba1 full length recombinant protein at 0.1 µg
    Lane 2 : HEK293 whole cell lysate at 30 µg
    Lane 3 : NIH3T3 whole cell lysate at 30 µg
    Lane 4 : HEK293 whole cell lysate at 30 µg
    Lane 5 : Human spleen tissue lysate at 30 µg
    Lane 6 : Mouse spleen tissue lysate at 30 µg
    Lane 7 : Rat spleen tissue lysate at 30 µg
    Lane 8 : U937 whole cell lysate at 30 µg
    Lane 9 : HL60 whole cell lysate at 30 µg
    Lane 10 : THP1 whole cell lysate at 30 µg
    Lane 11 : THP1 whole cell lysate, PMA treated at 30 µg
    Lane 12 : Raw 264.7 whole cell lysate at 30 µg
    Lane 13 : C6 whole cell lysate at 30 µg
    Lane 14 : NR8383 whole cell lysate at 30 µg

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 17 kDa
    Additional bands at : 17 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

    Lanes 1-14: Blocked in 3% milk for 1 hour (RT).

    Abcam recommends blocking in milk for cleaner blots with reduced background, in comparison to BSA. 

    This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab107159 (anti-Iba1 antibody; 2 ug/mL) for 18 hours at 4°C. Antibody binding was detected using a Dako HRP-labelled rabbit anti-goat IgG at  1:5,000 dilution for 1 hour at room temperature and visualised using ECL development solution ab133406.

  • ab107159 staining Iba1 in Rat liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 15 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2000 in PBS + 1% BSA) for 1 hour at 20°C. An undiluted HRP-conjugated anti-goat IgG polyclonal was used as the secondary antibody. Kupffer cells are positive for Iba-1.

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  • IHC-P image of Iba1 staining on human brain sections using ab107159 (1:2000). The paraffin embedded sectons were subjected to heat mediated antigen retrieval using citric acid. The sections was then blocked using 1% BSA at 21°C for 10 min. The secondary antibody was horse polyclonal to anti-sheep conjugated to biotin at 1:200.

    See Abreview

  • IHC-P image of Iba1 staining on mouse spleen sections using ab107159 (1:2000). The paraffin embedded sectons were subjected to heat mediated antigen retrieval using citric acid. The sections was then blocked using 1% BSA at 21°C for 10 min. The secondary antibody was horse polyclonal to anti-sheep conjugated to biotin at 1:200.

    See Abreview

  • IHC-P image of Iba1 staining on rat spleen sections using ab107159 (1:2000). The paraffin embedded sectons were subjected to heat mediated antigen retrieval using citric acid. The sections was then blocked using 1% BSA at 21°C for 10 min. The primary antibody was incubated at 21°C for 2 hours. The secondary antibody was goat polyclonal to anti-sheep conjugated to biotin at 1:250.

    See Abreview

Anti-Iba1 antibody (ab107159)参考文献

This product has been referenced in:
  • James RE  et al. Loss of galectin-3 decreases the number of immune cells in the subventricular zone and restores proliferation in a viral model of multiple sclerosis. Glia N/A:N/A (2015). Read more (PubMed: 26337870) »
  • Zinnhardt B  et al. Multimodal imaging reveals temporal and spatial microglia and matrix metalloproteinase activity after experimental stroke. J Cereb Blood Flow Metab 35:1711-21 (2015). IHC ; Mouse . Read more (PubMed: 26126867) »

See all 11 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry free floating
Sample Mouse Tissue sections (Brain)
Specification Brain
Username

Abcam user community

Verified customer

提交于 Apr 11 2016

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Spinal Cord)
Antigen retrieval step None
Permeabilization No
Specification Spinal Cord
Fixative Paraformaldehyde
Username

Chris Tsantoulas

Verified customer

提交于 Mar 17 2016

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain)
Antigen retrieval step None
Permeabilization Yes - triton 0.2%
Specification Brain
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative Paraformaldehyde
Username

Ms. Francoise Geffroy

Verified customer

提交于 Jul 28 2015

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (brain)
Antigen retrieval step None
Permeabilization No
Specification brain
Blocking step Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 20 · Temperature: 20°C
Fixative Paraformaldehyde
Username

Anat Boehm

Verified customer

提交于 Jul 14 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Pig Tissue sections (Small intestine)
Specification Small intestine
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Sep 11 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Marmoset (common) Tissue sections (Kidney)
Specification Kidney
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Feb 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Lung)
Specification Lung
Fixative Formaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Nov 05 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Spleen)
Specification Spleen
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Oct 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Spleen)
Specification Spleen
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Jun 14 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (Cerebellum)
Specification Cerebellum
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Jun 14 2012

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