Application
Western blot
Sample
Rat Cell lysate - whole cell (PC12)
Loading amount
30 µg
Specification
PC12
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk, 0.1% Tween-20, PBS
Secondary antibody
Name
Non-Abcam antibody was used: Swine anti rabbit-HRP
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 98 kDa Non-specific: 120 (Faint) kDa
Additional data
Additional Notes
In addition to detecting the putative full-length protein (98 kDa by SDS-PAGE), this antibody also recognises several lower molecular weight species. Since iASPP has at least two shorter isoforms, some of these bands may be specific but I haven't assigned them. Regardless of this, the antibody certainly recognises full-length rodent iASPP.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jun 22 2007