Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Anti-hypothetical protein FLJ30058 antibody 图像
Western blot - hypothetical protein FLJ30058 antibody (ab84010)
Anti-hypothetical protein FLJ30058 antibody (ab84010) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size :
62 kDaObserved band size :
55 kDa (why is the actual band size different from the predicted?
Anti-hypothetical protein FLJ30058 antibody (ab84010)参考文献
has not yet been referenced specifically in any publications.
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