Anti-HYPE抗体(ab67163)
Key features and details
- Mouse polyclonal to HYPE
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-HYPE抗体
参阅全部 HYPE 一抗 -
描述
小鼠多克隆抗体to HYPE -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human HYPE. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- Transfected 293T cell lysate, human placenta tissue lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab67163于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
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说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
靶标
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功能
Adenylyltransferase that mediates the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins. Able to inactivate Rho GTPases in vitro by adding AMP to RhoA, Rac and Cdc42. It is however unclear whether it inactivates GTPases in vivo and physiological substrates probably remain to be identified. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the fic family.
Contains 1 fido domain.
Contains 2 TPR repeats. -
结构域
The fido domain mediates the adenylyltransferase activity. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 11153 Human
- SwissProt: Q9BVA6 Human
- Unigene: 661891 Human
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别名
- Adenosine monophosphate-protein transferase FICD antibody
- AMPylator FICD antibody
- FIC domain containing antibody
see all
图片
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Anti-HYPE antibody (ab67163) at 1/500 dilution + human placenta tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa -
All lanes : Anti-HYPE antibody (ab67163) at 1/500 dilution
Lane 1 : Transfected 293T cell lysate
Lane 2 : non Transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
数据表及文件
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Datasheet download
文献 (1)
ab67163 被引用在 1 文献中.
- Sanyal A et al. Alpha-Synuclein Is a Target of Fic-Mediated Adenylylation/AMPylation: Possible Implications for Parkinson's Disease. J Mol Biol 431:2266-2282 (2019). PubMed: 31034889