Hydrogen Peroxide封闭试剂(ab64218)

概述

  • 产品名称
    Hydrogen Peroxide封闭试剂
  • 经测试应用
    适用于: IHC-P, IHC-Frmore details
  • 常规说明
    The concentration of this product is 0.3%.

    Immunohistochemistry
    Incubate tissue section for 5-10 minutes at room temperature prior to application of the primary antibody. After incubation rinse twice in buffer.

性能

应用

Our Abpromise guarantee covers the use of ab64218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.

文献

This product has been referenced in:
  • Selby MJ  et al. Preclinical Development of Ipilimumab and Nivolumab Combination Immunotherapy: Mouse Tumor Models, In Vitro Functional Studies, and Cynomolgus Macaque Toxicology. PLoS One 11:e0161779 (2016). Read more (PubMed: 27610613) »

See 1 Publication for this product

客户评价及客户问答

IHC-P with Ki67 antibody using H2O2 blocking reagant

Excellent Excellent 5/5 (Ease of Use)
Abreviews
H2O2 blocking reagant was used prior to protein blocking, and primary antibody was diluted 1:100 and incubated at RT for 1 hr. As the image indicates, the peroxidase activity was significantly blocked and there was minimal background staining.
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Abcam user community

Verified customer

提交于 Aug 16 2016

Hydrogen peroxidase Blocking Solution

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Using HRP conjugated antibody may result in high, non-specific background staining. The non-specific background can be significantly reduced by pre-treatment of tissues with hydrogen peroxide prior to incubation with HRP conjugated antibody. Pictures show mouse gastrocnemius staining with no pre-treatment and pre-treatment with Hydrogen Peroxide Blocking solution from abcam. Results shows a significant improvement with an abolished background staining.
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Dr. Francesco Elia Marino

Verified customer

提交于 Jul 23 2014


ab64218 and ab94666 are exactly the same products but ab64218 is sold as 125 ml and ab94666 is sold as 60 ml.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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