Hydrogen Peroxide封闭试剂(ab64218)

概述

  • 产品名称Hydrogen Peroxide封闭试剂
    参阅全部 Hydrogen peroxide blocking reagent 试剂
  • 经测试应用适用于: IHC-P, IHC-Frmore details
  • 常规说明The concentration of this product is 0.3%.

    Immunohistochemistry
    Incubate tissue section for 5-10 minutes at room temperature prior to application of the primary antibody. After incubation rinse twice in buffer.

性能

  • 相关性Hydrogen Peroxide Blocking Reagent is intended for use in peroxidase-based immunohistochemical staining procedures on cell preparations, frozen tissue sections, and paraffin-embedded tissue sections.
  • 别名
    • Hydrogen peroxide block

应用

Our Abpromise guarantee covers the use of ab64218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.

Hydrogen Peroxide Blocking Reagent (ab64218)参考文献

ab64218 has not yet been referenced specifically in any publications.

Product Wall

IHC-P with Ki67 antibody using H2O2 blocking reagant

Excellent Excellent 5/5 (Ease of Use)
Abreviews
H2O2 blocking reagant was used prior to protein blocking, and primary antibody was diluted 1:100 and incubated at RT for 1 hr. As the image indicates, the peroxidase activity was significantly blocked and there was minimal background staining.
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Abcam user community

Verified customer

提交于 Aug 16 2016

Hydrogen peroxidase Blocking Solution

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Using HRP conjugated antibody may result in high, non-specific background staining. The non-specific background can be significantly reduced by pre-treatment of tissues with hydrogen peroxide prior to incubation with HRP conjugated antibody. Pictures show mouse gastrocnemius staining with no pre-treatment and pre-treatment with Hydrogen Peroxide Blocking solution from abcam. Results shows a significant improvement with an abolished background staining.
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Dr. FRANCESCO ELIA MARINO

Verified customer

提交于 Jul 23 2014


ab64218 and ab94666 are exactly the same products but ab64218 is sold as 125 ml and ab94666 is sold as 60 ml.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"