概述

  • 产品名称Hydrogen Peroxide Assay试剂盒
    参阅全部 Hydrogen Peroxide 试剂盒
  • 测试
    200 x 1 assay
  • 样品类型
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • 检测类型Quantitative
  • 灵敏度
    > 0.04 µM
  • 检测时间
    1h 00m
  • 产品概述

    Hydrogen Peroxide Assay Kit (ab102500) is a highly sensitive, simple, direct and HTS-ready colorimetric and fluorometric assay for measuring H2O2 in biological samples. In the presence of Horse Radish Peroxidase (HRP), the OxiRed Probe reacts with H2O2 to produce product with color (?max = 570 nm) and red-fluorescent (Ex/Em=535/587 nm). The kit can perform 200 reactions by fluorometric method or 100 reactions by colorimetric method. The detection limit can be as low as 2 pmol per assay (or 40 nM concentration) of H2O2 in the sensitive fluorometric assay.


    Visit our FAQs page for tips and troubleshooting.

  • 说明

    Hydrogen Peroxide (H2O2) is a reactive oxygen metabolic byproduct that serves as a key regulator for a number of oxidative stress-related states. Functioning through NF-kappaB and other factors, hydroperoxide-mediated pathways have been linked to asthma, inflammatory arthritis, atherosclerosis, diabetic vasculopathy, osteoporosis, neuro-degenerative diseases, Down's syndrome and immune system diseases.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 相关性Hydrogen Peroxide (H2O2) is a reactive oxygen metabolic byproduct that serves as a key regulator for a number of oxidative stress-related states. Functioning through NF-kB and other factors, hydroperoxide-mediated pathways have been linked to asthma, inflammatory arthritis, atherosclerosis, diabetic vasculopathy, osteoporosis, neuro-degenerative diseases, Down’s syndrome and immune system diseases.
  • 别名
    • H2O2

应用

Our Abpromise guarantee covers the use of ab102500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

Hydrogen Peroxide Assay Kit 图像

  • H2O2 Colorimetric Standard Curve
  • H2O2 Fluorimetric Standard Curve

实验方案

Hydrogen Peroxide Assay Kit (ab102500)参考文献

This product has been referenced in:
  • Vicente CS  et al. Catalases Induction in High Virulence Pinewood Nematode Bursaphelenchus xylophilus under Hydrogen Peroxide-Induced Stress. PLoS One 10:e0123839 (2015). Read more (PubMed: 25894519) »
  • Wihastuti TA  et al. Vasa vasorum anti-angiogenesis through H2O2, HIF-1a, NF-?B, and iNOS inhibition by mangosteen pericarp ethanolic extract (Garcinia mangostana Linn) in hypercholesterol-diet-given Rattus norvegicus Wistar strain. Vasc Health Risk Manag 10:523-31 (2014). Read more (PubMed: 25187725) »

See all 5 Publications for this product

Product Wall

I can confirm that the kit ab102522 and ab102500 can be used to assay xanthine oxidase and Hydrogen peroxide in cells. I would suggest starting with at least 1-2 million cells. Please follow the following protocol;

2X106 cells, suspend the ce...

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As recommended on the protocol, an amount ranging from 2 to 50ul should be added to each well, and fill it up to 50ul with assay buffer.

As the concentration of H2O2 in your samples is unknown, you may need to run a quick test before...

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The kit ab102500 is suitable with many type of biological fluids and extracts such as cell lysates and tissue culture supernatant.

Frozen sample can be used with this kit so once the extract is prepared these should be immediately frozen at -...

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Thank you for contacting us.
Since we mention only a 10 min incubation time for this reaction, I would recommend taking the reading right after. Waiting for more time might result in increase in the background signal and later drop in the total si...

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Thank you for contacting us.

Please buy the requested product ab167452.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

I have sent a request to my colleague for adding this product in catalogue. I will let you know the catalogue number soon.

Thanks!

Thank you for contacting us.

We can provide one bottle of assay buffer for £90. Please let us know if you are interested in buying.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any ...

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Thank you for contacting us.
This kit can actually be used both colorimetrically and fluorometrically. In general fluorometric analysis is ˜10 times more sensitive than colorimetric analysis. The detection limit with this assay can be as low as 2 ...

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We recommend the use of the 10 kDa filters irrespective of your immediate or later usage of the lysed samples. The filters help in separating out proteins which can chew up the analyte. You most likely will not get efficient results without the fliters...

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Thank you for contacting us.

You can use the H2O2 assay buffer in the kit as the lysis buffer for cells.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"