Anti-SPAM1抗体(ab196596)
Key features and details
- Rabbit polyclonal to SPAM1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-SPAM1抗体
参阅全部 SPAM1 一抗 -
描述
兔多克隆抗体to SPAM1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment within Human SPAM1 (internal sequence). The exact sequence is proprietary.
Database link: P38567 -
阳性对照
- Hela cells, extracts of PC3, Raji, BT474 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride
without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab196596于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/50 - 1/200.
|
|
WB |
1/500 - 1/2000. Predicted molecular weight: 58 kDa.
|
说明 |
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ICC/IF
1/50 - 1/200. |
WB
1/500 - 1/2000. Predicted molecular weight: 58 kDa. |
靶标
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功能
Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid. -
组织特异性
Testis. -
序列相似性
Belongs to the glycosyl hydrolase 56 family. -
翻译后修饰
N-glycosylated. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6677 Human
- Omim: 600930 Human
- SwissProt: P38567 Human
- Unigene: 121494 Human
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别名
- epididymis secretory sperm binding protein Li 96n antibody
- HEL-S-96n antibody
- HYA1 antibody
see all
图片
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab196596 被引用在 4 文献中.
- Biering SB et al. SARS-CoV-2 Spike triggers barrier dysfunction and vascular leak via integrins and TGF-β signaling. Nat Commun 13:7630 (2022). PubMed: 36494335
- Xiong X et al. Co-expression of IL-7 and PH20 promote anti-GPC3 CAR-T tumour suppressor activity in vivo and in vitro. Liver Int 41:1033-1043 (2021). PubMed: 33347692
- Biering SB et al. SARS-CoV-2 Spike triggers barrier dysfunction and vascular leak via integrins and TGF-β signaling. bioRxiv N/A:N/A (2021). PubMed: 34931188
- Hong Y et al. Degradation of tumour stromal hyaluronan by small extracellular vesicle-PH20 stimulates CD103+ dendritic cells and in combination with PD-L1 blockade boosts anti-tumour immunity. J Extracell Vesicles 8:1670893 (2019). PubMed: 31632619