Anti-Hyaluronic acid抗体(ab53842)


  • 产品名称
    Anti-Hyaluronic acid抗体
  • 描述
    羊多克隆抗体to Hyaluronic acid
  • 宿主
  • 特异性
    ab53842 is specific for Hyaluronic acid, a mucopolysaccharide which is an integral part of the gel-like substance of animal connective tissue that serves as a lubricating and protective agent.
  • 经测试应用
    适用于: ELISA, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Chicken, Dog, Human
    预测可用于: a wide range of other species
  • 免疫原

    Hyaluronic acid from human umbilical cord


  • 形式
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
  • 同种型
  • 研究领域


Our Abpromise guarantee covers the use of ab53842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

See Abreview.

IHC-Fr Use at an assay dependent concentration.

See Abreview.


  • 相关性
    Hyaluronic acid is a mucopolysaccharide which is an integral part of the gel-like substance of animal connective tissue that serves as a lubricanting and protective agent.


  • ab53842 staining Hyaluronic acid in chicken cockscomb tissue sections by Immunohistochemistry (formaldehyde fixed sections). Tissue was fixed with formaldehyde and an enzymatic antigen retrieval step was performed using Pronase. Samples were then blocked with 3% H2O2 for 5 minutes at 20°C followed by incubation with the primary antibody at a 1/300 dilution for 1 hour at 20°C. ab6747 Rabbit polyclonal to Sheep IgG - H&L (HRP) was used as secondary antibody, undiluted.

    See Abreview

  • Immunohistochemical analysis of acetone-fixed 10 µm cryostat cut human eplileptic substantia alba tissue, labelling hyaluronic acid with ab53842, incubated at a dilution of 1/250 for 1 hour at 37°C in TNB diluent. Blocking was using a TNB blocking buffer for 30 minutes at 25°C. The secondary used was a polyclonal Alexa Fluor® 488 conjugate used at 1/250.

    See Abreview


This product has been referenced in:
  • Voutouri C & Stylianopoulos T Accumulation of mechanical forces in tumors is related to hyaluronan content and tissue stiffness. PLoS One 13:e0193801 (2018). Read more (PubMed: 29561855) »
  • Ryu YA  et al. Histological, Physical Studies after Xenograft of Porcine Ear Cartilage. Arch Craniofac Surg 18:155-161 (2017). Read more (PubMed: 29090195) »

See all 16 Publications for this product


Thank you for your reply.

I am not sure what you mean by "revealed system." The secondary antibody used to generate the image on our datasheet was Rabbit polyclonal to Sheep IgG - H&L (HRP) such as ab6747, and it was used with D...

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (CNS tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid buffer
Yes - Triton X
CNS tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C

Dr. Martin Broadstock

Verified customer

提交于 Feb 02 2016

We have not tested this antibody against hyaluronic acid which has been degraded by streptococcal hyaluronidase. The immunogen used was highly polymerized human hyaluronic acid as potassium salt isolated from human umbilical cord containing <2% prot...

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Thank you for your updates.
I am very pleased to hear that finally after some optimization steps this antibody works in IHC as it is expected. Thank you for sharing your feedback with us.
If you need any further assistance in the future, plea...

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Thank you for your response.
Normally we do not send a free vial if our customer has run out of the antibody. However, this is a special case and I understand your concern so take your request as an exemption.
This is to let you know that I h...

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Thank you for your enquiry regarding ab53842 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.
I would like to reassure you that our Abpromise applie...

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Thank you for your patience.

The immunogen used for this antibody was product H1504 from Sigma Aldrich. The antibody binds simply because hyaluronic acid is antigenic; it is the same principle for how antibodies naturally bind bacteria (ie the...

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I am sorry to hear that you have been experiencing problems using this product in the application that you wish.
I would like to reassure you that our Abpromise applies to your complaint since you purchased this product within the guarantee period...

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Thank you for contacting us yesterday and your interest in our products.

As discussed over the phone, the anti-Hyaluronic acid antibody (ab53842) has not as yet been used in ICC as you are hoping to do. This does not mean that it would not wo...

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Thank you for your email.

It is possible to raise antibodies to hyaluronic acid in the absence of a carrier protein. This is because hyaluronic acid is a high molecular weight polysaccharide. Such polymers typically have sufficient antigenicit...

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