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Hyaluronic acid from human umbilical cord
Our Abpromise guarantee covers the use of ab53842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.
|IHC-Fr||Use at an assay dependent concentration.
Immunohistochemical analysis of acetone-fixed 10 µm cryostat cut human eplileptic substantia alba tissue, labelling hyaluronic acid with ab53842, incubated at a dilution of 1/250 for 1 hour at 37°C in TNB diluent. Blocking was using a TNB blocking buffer for 30 minutes at 25°C. The secondary used was a polyclonal Alexa Fluor® 488 conjugate used at 1/250.