Anti-HYAL3抗体(ab67248)

概述

  • 产品名称Anti-HYAL3抗体
    参阅全部 HYAL3 一抗
  • 描述
    小鼠多克隆抗体to HYAL3
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length HYAL3 protein (Human)

  • 阳性对照
    • Hela cells. HYAL3 transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab67248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ICC/IF
  • 应用说明ICC/IF: Use at a concentration of 10 µg/ml.
    WB: 1/500 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).

    NOTE: This product requires purification with Protein A prior to use in ICC/IF.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 组织特异性Bone marrow, testis and kidney. Isoform 4 is detected in all bladder tumor and prostate tumor cells.
    • 序列相似性Belongs to the glycosyl hydrolase 56 family.
      Contains 1 EGF-like domain.
    • 细胞定位Secreted. Lysosome.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Hyal-3 antibody
      • HYAL3 antibody
      • HYAL3_HUMAN antibody
      • Hyaluronidase 3 antibody
      • Hyaluronidase-3 antibody
      • Hyaluronoglucosaminidase 3 antibody
      • Hyaluronoglucosaminidase-3 antibody
      • LUCA-3 antibody
      • LUCA14 antibody
      • LUCA3 antibody
      • Lung carcinoma protein 3 antibody
      • Minna14 antibody
      see all

    Anti-HYAL3 antibody 图像

    • All lanes : Anti-HYAL3 antibody (ab67248) at 1/500 dilution

      Lane 1 : HYAL3 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg/ml per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 47 kDa
      Observed band size : 47 kDa
    • Immunofluorescent staining of HYAL3 in HeLa cells using purified ab67248 at 10µg/ml.

    Anti-HYAL3 antibody (ab67248)参考文献

    ab67248 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing
    Sample Human Cell lysate - whole cell (fibroblast)
    Specification fibroblast
    Treatment 10ng/ml IL-1beta 24hrs
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    Verified customer

    提交于 Feb 26 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"