概述

  • 产品名称Anti-HYAL1抗体
    参阅全部 HYAL1 一抗
  • 描述
    兔多克隆抗体to HYAL1
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (WNANTQWCLE RHGVDVDVSV FDVVANPGQT FRGPDMTIFY SSQLGTYPYY) of Human HYAL1 (NP_695014)

  • 阳性对照
    • HepG2 cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab85375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能May have a role in promoting tumor progression. May block the TGFB1-enhanced cell growth.
  • 组织特异性Highly expressed in the liver, kidney and heart. Weakly expressed in lung, placenta and skeletal muscle. No expression detected in adult brain. Isoform 1 is expressed only in bladder and prostate cancer cells, G2/G3 bladder tumor tissues and lymph node specimens showing tumor invasive tumors cells. Isoform 3, isoform 4, isoform 5 and isoform 6 are expressed in normal bladder and bladder tumor tissues.
  • 疾病相关Defects in HYAL1 are the cause of mucopolysaccharidosis type 9 (MPS9) [MIM:601492]; also called hyaluronidase deficiency. MPS9 is a lysosomal storage disease characterized by high hyaluronan (HA) concentration in the serum. The clinical features are periarticular soft tissue masses, mild short stature and acetabular erosions, and absence of neurological or visceral involvement.
  • 序列相似性Belongs to the glycosyl hydrolase 56 family.
    Contains 1 EGF-like domain.
  • 细胞定位Secreted. Lysosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HYAL 1 antibody
    • Hyal-1 antibody
    • HYAL1 antibody
    • HYAL1_HUMAN antibody
    • Hyaluronidase 1 antibody
    • Hyaluronidase-1 antibody
    • Hyaluronoglucosaminidase 1 antibody
    • Hyaluronoglucosaminidase-1 antibody
    • Hyaluronoglucosaminidase1 antibody
    • LUCA 1 antibody
    • LUCA-1 antibody
    • LUCA1 antibody
    • Lung carcinoma protein 1 antibody
    • MGC45987 antibody
    • NAT6 antibody
    • Plasma hyaluronidase antibody
    • Tumor suppressor LUCA 1 antibody
    see all

Anti-HYAL1 antibody 图像

  • Anti-HYAL1 antibody (ab85375) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 48 kDa
  • ab85375 staining HYAL1 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Anti-HYAL1 antibody (ab85375)参考文献

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See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (orbital fibroblast)
Specification orbital fibroblast
Treatment 10 ng/ml IL-1beta
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Feb 25 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"