Anti-HVCN1抗体(ab76847)

概述

  • 产品名称Anti-HVCN1抗体
    参阅全部 HVCN1 一抗
  • 描述
    兔多克隆抗体to HVCN1
  • 经测试应用适用于: IHC-Fr, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from N terminal domain of human HVCN1 protein.

性能

应用

Our Abpromise guarantee covers the use of ab76847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/50 - 1/200.
WB 1/100 - 1/1000. Predicted molecular weight: 32 kDa.

靶标

  • 功能Mediates the voltage-dependent proton permeability of excitable membranes. Forms a proton-selective channel through which protons may pass in accordance with their electrochemical gradient. Proton efflux, accompanied by membrane depolarization, facilitates acute production of reactive oxygen species in phagocytosis.
  • 组织特异性Enriched in immune tissues, such as lymph nodes, B-lymphocytes, monocytes and spleen.
  • 序列相似性Belongs to the hydrogen channel family.
  • 结构域The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. Unlike other voltage-gated ion channels it lacks the pore domain.
    The C-terminal coiled coil region mediates homodimerization and cooperative channel gating. It is essential for normal subcellular localization.
  • 翻译后修饰Phosphorylation may enhance channel gating.
  • 细胞定位Membrane. Cell membrane. Detected mainly at intracellular membranes upon overexpression in HeLa cells (PuMed:20147290), but not in other cell types.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HV 1 antibody
    • HV1 antibody
    • HVCN 1 antibody
    • HVCN1 antibody
    • HVCN1_HUMAN antibody
    • Hydrogen voltage gated channel 1 antibody
    • Hydrogen voltage-gated channel 1 antibody
    • Voltage gated hydrogen channel 1 antibody
    • Voltage sensor domain only protein antibody
    • Voltage sensor domain-only protein antibody
    • Voltage-gated hydrogen channel 1 antibody
    • VSOP antibody
    see all

Anti-HVCN1 antibody (ab76847)参考文献

ab76847 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (PLB-985, a subclone of HL-60)
Loading amount 1e+006 cells
Specification PLB-985, a subclone of HL-60
Treatment DMFA (5% v/v) for 5 days
Gel Running Conditions Reduced Denaturing (10%)
Blocking step skimmed milk powder (+ 0.1% normal goat serum) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 May 18 2010

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