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RabMAb

Anti-HUS1抗体[EPR5132] (ab109371)

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概述

  • 产品名称
    Anti-HUS1抗体[EPR5132]
    参阅全部 HUS1 一抗
  • 描述
    兔单克隆抗体[EPR5132] to HUS1
  • 经测试应用
    适用于: WB, IP, IHC-P, Flow Cytmore details
    不适用于: ICC
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human HUS1.

  • 阳性对照
    • HeLa, K562, and A431 cell lysates, Human breast carcinoma tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab109371 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 32 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明
    Is unsuitable for ICC.

    • 靶标

    • 功能
      Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates.
    • 组织特异性
      Ubiquitous.
    • 序列相似性
      Belongs to the HUS1 family.
    • 细胞定位
      Nucleus. Cytoplasm. In discrete nuclear foci upon DNA damage. According to PubMed:14500360, localized also in the cytoplasm. DNA damage induces its nuclear translocation. Shuttles between the nucleus and the cytoplasm.
    • Information by UniProt
    • 数据库链接
      • 别名
        • Checkpoint protein HUS1 antibody
        • hHUS1 antibody
        • HUS1 (S. pombe) checkpoint homolog antibody
        • Hus1 antibody
        • HUS1 checkpoint homolog (S. pombe) antibody
        • HUS1 Checkpoint Protein antibody
        • HUS1 protein antibody
        • HUS1+ - like protein antibody
        • HUS1_HUMAN antibody
        • Hydroxyurea-sensitive 1, S. pombe, homolog of antibody
        see all

      Anti-HUS1 antibody [EPR5132] 图像

      • Flow Cytometry - Anti-HUS1 antibody [EPR5132] (ab109371)
        Flow Cytometry - Anti-HUS1 antibody [EPR5132] (ab109371)
        Overlay histogram showing HeLa cells stained with ab109371 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109371, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
      • Western blot - HUS1 antibody [EPR5132] (ab109371)
        Western blot - HUS1 antibody [EPR5132] (ab109371)
        All lanes : Anti-HUS1 antibody [EPR5132] (ab109371) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : K562 cell lysate
        Lane 3 : A431 cell lysate

        Lysates/proteins at 10 µg per lane.


        Predicted band size : 32 kDa
        Observed band size : 34 kDa (why is the actual band size different from the predicted?)
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HUS1 antibody [EPR5132] (ab109371)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HUS1 antibody [EPR5132] (ab109371)
        Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using ab109371 at a dilution of 1/100.

      Anti-HUS1 antibody [EPR5132] (ab109371)参考文献

      ab109371 has not yet been referenced specifically in any publications.

      发表研究结果有使用 ab109371？请让我们知道，以便我们可以引用本数据表中的参考文章。

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