概述
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产品名称Anti-HUS1抗体[EPR5132]
参阅全部 HUS1 一抗 -
描述兔单克隆抗体[EPR5132] to HUS1
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宿主Rabbit
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经测试应用适用于: WB, IP, IHC-P, Flow Cytmore details
不适用于: ICC -
种属反应性与反应: Human
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免疫原
Synthetic peptide corresponding to residues in Human HUS1.
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阳性对照
- HeLa, K562, and A431 cell lysates, Human breast carcinoma tissue
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常规说明
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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纯度Tissue culture supernatant
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克隆单克隆
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克隆编号EPR5132
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同种型IgG
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研究领域
相关产品
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Positive Controls
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Related Products
应用
Our Abpromise guarantee covers the use of ab109371 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 32 kDa). | |
| IP | 1/10 - 1/100. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| Flow Cyt | 1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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靶标
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功能Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates.
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组织特异性Ubiquitous.
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序列相似性Belongs to the HUS1 family.
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细胞定位Nucleus. Cytoplasm. In discrete nuclear foci upon DNA damage. According to PubMed:14500360, localized also in the cytoplasm. DNA damage induces its nuclear translocation. Shuttles between the nucleus and the cytoplasm.
- Information by UniProt
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数据库链接
- Entrez Gene: 3364 Human
- Omim: 603760 Human
- SwissProt: O60921 Human
- Unigene: 152983 Human
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别名
- Checkpoint protein HUS1 antibody
- hHUS1 antibody
- HUS1 (S. pombe) checkpoint homolog antibody
see all
图片
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![Flow Cytometry - Anti-HUS1 antibody [EPR5132] (ab109371)](http://www.abcam.cn/ps/products/109/ab109371/Images/ab109371-3-ab109371FC.jpg)
Flow Cytometry - Anti-HUS1 antibody [EPR5132] (ab109371)Overlay histogram showing HeLa cells stained with ab109371 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109371, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. -
![Western blot - Anti-HUS1 antibody [EPR5132] (ab109371)](http://www.abcam.cn/ps/products/109/ab109371/Images/HUS1-Primary-antibodies-ab109371-3.JPG)
Western blot - Anti-HUS1 antibody [EPR5132] (ab109371)All lanes : Anti-HUS1 antibody [EPR5132] (ab109371) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 32 kDa
Observed band size: 34 kDa (why is the actual band size different from the predicted?) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HUS1 antibody [EPR5132] (ab109371)](http://www.abcam.cn/ps/products/109/ab109371/Images/HUS1-Primary-antibodies-ab109371-4.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HUS1 antibody [EPR5132] (ab109371)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using ab109371 at a dilution of 1/100.
实验方案
文献
ab109371 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"