Anti-HURP抗体(ab84509)
Key features and details
- Rabbit polyclonal to HURP
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-HURP抗体
参阅全部 HURP 一抗 -
描述
兔多克隆抗体to HURP -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human HURP aa 200-300.
Database link: Q15398 -
阳性对照
- IHC-P: Human basal cell carcinoma and human seminoma tissues.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab84509于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/100 - 1/500.
|
|
WB |
1/200.
|
说明 |
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IHC-P
1/100 - 1/500. |
WB
1/200. |
靶标
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功能
Potential cell cycle regulator that may play a role in carcinogenesis of cancer cells. Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells. -
组织特异性
Abundantly expressed in fetal liver. Expressed at lower levels in bone marrow, testis, colon, and placenta. -
序列相似性
Belongs to the SAPAP family. -
发展阶段
Elevated levels of expression detected in the G2/M phase of synchronized cultures of HeLa cells. -
翻译后修饰
Ubiquitinated, leading to its degradation.
Phosphorylated upon DNA damage, probably by ATM or ATR. Decreased phosphorylation levels are associated with the differentiation of intestinal epithelial cells. -
细胞定位
Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Localizes to the spindle in mitotic cells. Colocalizes with CDH1 at sites of cell-cell contact in intestinal epithelial cells. - Information by UniProt
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数据库链接
- Entrez Gene: 9787 Human
- SwissProt: Q15398 Human
- Unigene: 77695 Human
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别名
- DAP-5 antibody
- DAP5 antibody
- Discs large homolog 7 antibody
see all
图片
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ab84509, at 1/250 dilution, staining HURP in formalin-fixed, paraffin-embedded human basal cell carcinoma by Immunohistochemistry, using DAB staining.
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ab84509, at 1/250 dilution, staining HURP in formalin-fixed, paraffin-embedded human seminoma by Immunohistochemistry, using DAB staining.
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Anti-HURP antibody (ab84509) at 1/200 dilution + 25µg of WCE from U20S cells synchronized by thimidine arrest and release to enrich in mitotic cells (same on lane 1 and 2).
Image kindly provided by Patrizia Lavia, CNR National Research Council, University "La Sapienza", Rome, Italy.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab84509 被引用在 3 文献中.
- Zhang X et al. Nucleolar and Spindle Associated Protein 1 (NUSAP1) Inhibits Cell Proliferation and Enhances Susceptibility to Epirubicin In Invasive Breast Cancer Cells by Regulating Cyclin D Kinase (CDK1) and DLGAP5 Expression. Med Sci Monit 24:8553-8564 (2018). PubMed: 30476929
- Espinoza I et al. Hypoxia on the Expression of Hepatoma Upregulated Protein in Prostate Cancer Cells. Front Oncol 6:144 (2016). IHC . PubMed: 27379206
- Ma MP et al. Sorting nexin 9 recruits clathrin heavy chain to the mitotic spindle for chromosome alignment and segregation. PLoS One 8:e68387 (2013). PubMed: 23861900