概述

  • 产品名称Anti-HURP抗体
    参阅全部 HURP 一抗
  • 描述
    兔多克隆抗体to HURP
  • 经测试应用适用于: IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to a region between residue 200 and 250 of Human HURP (NP_055565.2).

  • 阳性对照
    • HeLa and 293T whole cell lysates.

性能

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应用

Our Abpromise guarantee covers the use of ab70743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 94 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Potential cell cycle regulator that may play a role in carcinogenesis of cancer cells. Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells.
  • 组织特异性Abundantly expressed in fetal liver. Expressed at lower levels in bone marrow, testis, colon, and placenta.
  • 序列相似性Belongs to the SAPAP family.
  • 发展阶段Elevated levels of expression detected in the G2/M phase of synchronized cultures of HeLa cells.
  • 翻译后修饰Ubiquitinated, leading to its degradation.
    Phosphorylated upon DNA damage, probably by ATM or ATR. Decreased phosphorylation levels are associated with the differentiation of intestinal epithelial cells.
  • 细胞定位Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Localizes to the spindle in mitotic cells. Colocalizes with CDH1 at sites of cell-cell contact in intestinal epithelial cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DAP-5 antibody
    • DAP5 antibody
    • Discs large homolog 7 antibody
    • Disks large associated protein 5 antibody
    • Disks large associated protein DLG7 antibody
    • Disks large-associated protein 5 antibody
    • Disks large-associated protein DLG7 antibody
    • DLG 1 antibody
    • DLG 7 antibody
    • DLG1 antibody
    • DLG7 antibody
    • DLGAP5 antibody
    • DLGP5_HUMAN antibody
    • Drosophila discs large 1 tumor suppressor like antibody
    • Hepatoma up regulated protein antibody
    • Hepatoma up-regulated protein antibody
    • Hepatoma upregulated protein antibody
    • HURP antibody
    • KIAA0008 antibody
    see all

Anti-HURP antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling HURP with ab70743 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-HURP antibody (ab70743) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 94 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • Detection of HURP by Western Blot of Immunprecipitate. ab70743 at 1µg/ml staining HURP in HeLa whole cell lysate immunoprecipitated using ab70743 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.

Anti-HURP antibody (ab70743)参考文献

ab70743 has not yet been referenced specifically in any publications.

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