概述

  • 产品名称
    Anti-HuR / ELAVL1抗体
    参阅全部 HuR / ELAVL1 一抗
  • 描述
    小鼠单克隆抗体to HuR / ELAVL1
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: MSNGYEDHMA EDCRGDIGRT NLIVNYLPQN MTQDELRSLF SSIGEVESAK LIRDKVAGHS LGYGFVNYVT AKDAERAINT LNGLRLQSKT IKVSYARPSS , corresponding to amino acids 1-101 of Human HuR/ ELAVL1

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

性能

应用

Our Abpromise guarantee covers the use of ab54987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明
    WB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.
    • 组织特异性
      Ubiquitous.
    • 序列相似性
      Belongs to the RRM elav family.
      Contains 3 RRM (RNA recognition motif) domains.
    • 翻译后修饰
      Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge.
    • 细胞定位
      Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ELAV (embryonic lethal abnormal vision Drosophila) like 1 antibody
      • ELAV (embryonic lethal, abnormal vision, Drosophila) like 1 (Hu antigen R) antibody
      • ELAV like 1 antibody
      • ELAV like RNA binding protein 1 antibody
      • ELAV-like protein 1 antibody
      • ELAV1 antibody
      • ELAV1_HUMAN antibody
      • Elavl1 antibody
      • Embryonic lethal abnormal vision drosophila homolog like 1 antibody
      • Hu Antigen R antibody
      • Hu-antigen R antibody
      • Hua antibody
      • HuR antibody
      • MelG antibody
      see all

    Anti-HuR / ELAVL1 antibody 图像

    • ab54987 Immunoprecipitating HuR / ELAVL1 in Human lymphocyte cell lysate. 700µg of cell lysate was incubated with primary antibody (5 µg/mg in abcam non-denaturing buffer) and matrix (Protein G) for 2 hours at 4°C. For western blot, ab54987 (1/1000) was used to confirm successful immunoprecipitation. Lane 1 - 25μg of lysate pre-IP, Lanes 2 and 3 -15μg of 70μg elution from matrix. 4-12% Bis-Tris gel.

      See Abreview



    • Predicted band size : 36 kDa
      HuR / ELAVL1 antibody (ab54987) at 1ug/lane + HeLa cell lysate at 25ug/lane.

    Anti-HuR / ELAVL1 antibody (ab54987)参考文献

    This product has been referenced in:
    • Chai Y  et al. HuR-regulated lncRNA NEAT1 stability in tumorigenesis and progression of ovarian cancer. Cancer Med 5:1588-98 (2016). WB ; Human . Read more (PubMed: 27075229) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunoprecipitation
    Immuno-precipitation step
    Protein G
    Sample
    Human Cell lysate - whole cell (lymphocytes)
    Specification
    lymphocytes
    Total protein in input
    700 µg
    Username

    Abcam user community

    Verified customer

    提交于 Aug 30 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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