概述

  • 产品名称
    Anti-HuR / ELAVL1抗体
    参阅全部 HuR / ELAVL1 一抗
  • 描述
    兔多克隆抗体to HuR / ELAVL1
  • 经测试应用
    适用于: IHC-FoFr, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Chicken, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human HuR/ ELAVL1.

    (Peptide available as ab28843.)

  • 阳性对照
    • HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate, Jurkat(Human T cell lymphoblast-like cell line) Whole Cell Lysate and A431 (Human epithelial carcinoma cell line) Whole Cell Lysate.

性能

应用

Our Abpromise guarantee covers the use of ab28660 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr 1/300.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). Another band of approximately 52 kDa is also detected, we are unsure as the identity of this band.

靶标

  • 功能
    Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.
  • 组织特异性
    Ubiquitous.
  • 序列相似性
    Belongs to the RRM elav family.
    Contains 3 RRM (RNA recognition motif) domains.
  • 翻译后修饰
    Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ELAV (embryonic lethal abnormal vision Drosophila) like 1 antibody
    • ELAV (embryonic lethal, abnormal vision, Drosophila) like 1 (Hu antigen R) antibody
    • ELAV like 1 antibody
    • ELAV like RNA binding protein 1 antibody
    • ELAV-like protein 1 antibody
    • ELAV1 antibody
    • ELAV1_HUMAN antibody
    • Elavl1 antibody
    • Embryonic lethal abnormal vision drosophila homolog like 1 antibody
    • Hu Antigen R antibody
    • Hu-antigen R antibody
    • Hua antibody
    • HuR antibody
    • MelG antibody
    see all

图片

  • All lanes : Anti-HuR / ELAVL1 antibody (ab28660) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : A431 whole cell lysate (ab7909)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Observed band size : 36 kDa
    Additional bands at : 52 kDa. We are unsure as to the identity of these extra bands.

文献

This product has been referenced in:

See 1 Publication for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
No
Username

Dr. Sophie Pezet

Verified customer

提交于 Feb 08 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NSC34)
Loading amount
10 µg
Specification
NSC34
Gel Running Conditions
Reduced Denaturing (4-12% nupage gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 24 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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