这个 快线抗体 还未被充份测试和鉴定,因此它受制于相关的 使用条款

Anti-Huntingtin (phospho S421)抗体(ab2174)

概述

  • 产品名称
    Anti-Huntingtin (phospho S421)抗体
    参阅全部 Huntingtin 一抗
  • 描述
    兔多克隆抗体to Huntingtin (phospho S421)

    该产品是 快线抗体,通过亲和纯化得到。对抗原肽有高灵敏度及特异性(ELISA测试)。 Read the terms of use »

  • 特异性
    Detects a 190kDa band in PC-3 cells, which may correspond to Huntingtin. Stains neuronal cytoplasm in immunohistochemistry (the expected localisation). The antibody has not been conclusively tested for specificity for phospho S421 Huntingtin.
  • 种属反应性

    预测可用于: Human, Pig
  • 免疫原

    Synthetic peptide: RSRSGpSIVE, corresponding to amino acids 416-424 of Human Huntingtin

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, 0.01% Sodium Azide, pH 7.2
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

应用 Ab评论 说明
ELISA Use at an assay dependent concentration. This antibody gave a positive result in ELISA against the immunizing peptide .
IHC-Fr 1/250.

靶标

  • 功能
    May play a role in microtubule-mediated transport or vesicle function.
  • 组织特异性
    Expressed in the brain cortex (at protein level). Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.
  • 疾病相关
    Defects in HTT are the cause of Huntington disease (HD) [MIM:143100]. HD is an autosomal dominant neurodegenerative disorder characterized by involuntary movements (chorea), general motor impairment, psychiatric disorders and dementia. Onset of the disease occurs usually in the third or fourth decade of life and symptoms progressively worsen leading to death in 10 to 20 years. Onset and clinical course depend on the degree of poly-Gln repeat expansion, longer expansions resulting in earlier onset and more severe clinical manifestations. HD affects 1 in 10,000 individuals of European origin. Neuropathology of Huntington disease displays a distinctive pattern with loss of neurons, especially in the caudate and putamen (striatum).
  • 序列相似性
    Belongs to the huntingtin family.
    Contains 10 HEAT repeats.
  • 结构域
    The N-terminal Gln-rich and Pro-rich domain has great conformational flexibility and is likely to exist in a fluctuating equilibrium of alpha-helical, random coil, and extended conformations.
  • 翻译后修饰
    Cleaved by apopain downstream of the polyglutamine stretch. The resulting N-terminal fragment is cytotoxic and provokes apoptosis.
    Forms with expanded polyglutamine expansion are specifically ubiquitinated by SYVN1, which promotes their proteasomal degradation.
  • 细胞定位
    Cytoplasm. Nucleus. The mutant Huntingtin protein colocalizes with AKAP8L in the nuclear matrix of Huntington's disease neurons.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AI256365 antibody
    • C430023I11Rik antibody
    • HD antibody
    • HD protein antibody
    • HD_HUMAN antibody
    • HDH antibody
    • HTT antibody
    • Huntingtin antibody
    • HUNTINGTON CHOREA antibody
    • Huntington disease protein antibody
    • Huntington's disease protein homolog antibody
    • IT 15 antibody
    • IT15 antibody
    • OTTMUSP00000026909 antibody
    • ZHD antibody
    see all

图片

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • Anti-Huntingtin (phospho S421) antibody (ab2174) at 1/1000 dilution + human PC-3 cell lysate

    Observed band size : 190 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 120 kDa,130 kDa. We are unsure as to the identity of these extra bands.
  • ab2174 was used at a 1:100 dilution to detect phosphorylated Huntingtin by immunohistochemistry in human brain cerebellum. Positive cytoplasmic staining is observed in neurons. Tissue was formalin-fixed and paraffin embedded. Detection was with AEC (pink), nuclear counterstaining with Haemotoxylin (blue).

文献

ab2174 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Permeabilization
Yes - 1% Triton X-100 in TBS
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Mr. Marian Hruska-Plochan

Verified customer

提交于 Oct 25 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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