Anti-Huntingtin Associated蛋白1抗体[1B6] (ab39713)
Key features and details
- Mouse monoclonal [1B6] to Huntingtin Associated Protein 1
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG1
概述
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产品名称
Anti-Huntingtin Associated蛋白1抗体[1B6] -
描述
小鼠单克隆抗体[1B6] to Huntingtin Associated蛋白1 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat
预测可用于: Monkey -
免疫原
Fusion protein. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- ab39713 gave a strong signal in Mouse Brain Tissue Lysate. The same band was detected in Rat Brain Tissue Lysate, although at a lower intensity.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
1B6 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab39713于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
说明 |
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Immunohistochemistry (PFA fixed): Use at an assay dependent dilution.
IHC-FrFl: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: 1/100 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 75 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
Associates specifically with huntingtin. This binding is enhanced by an expanded polyglutamine repeat. -
组织特异性
Predominantly expressed in brain. Selectively expressed in neurons. -
序列相似性
Contains 1 HAP1 N-terminal domain. - Information by UniProt
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数据库链接
- Entrez Gene: 15114 Mouse
- Entrez Gene: 29430 Rat
- SwissProt: O35668 Mouse
- SwissProt: P54256 Rat
- Unigene: 281700 Mouse
- Unigene: 37430 Rat
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别名
- HAP 1 antibody
- HAP 1A antibody
- HAP 2 antibody
see all
图片
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All lanes : Anti-Huntingtin Associated Protein 1 antibody [1B6] (ab39713) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate - normal tissue
Lane 2 : Brain (Mouse) Tissue Lysate - normal tissue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 75 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa (possible non-specific secondary antibody binding)
ab39713 detects a band at 85 kDa in mouse and rat brain tissue lysate. This band corresponds to the size of the HAP1-B protein detected in rat brain tissue lysate by Shi-Hua Li et al. (1998). (PMID: 9798945).
数据表及文件
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Datasheet download
文献 (3)
ab39713 被引用在 3 文献中.
- Torre ER et al. A protocol for isolation and biochemical characterization of stigmoid bodies from rat brain. J Neurosci Methods 125:27-32 (2003). PubMed: 12763227
- Gutekunst CA et al. Stigmoid bodies contain type I receptor proteins SorLA/LR11 and sortilin: new perspectives on their function. J Histochem Cytochem 51:841-52 (2003). PubMed: 12754295
- Chan EY et al. Targeted disruption of Huntingtin-associated protein-1 (Hap1) results in postnatal death due to depressed feeding behavior. Hum Mol Genet 11:945-59 (2002). PubMed: 11971876