人VEGF Receptor 3 ELISA试剂盒(ab100666)

概述

  • 产品名称人VEGF Receptor 3 ELISA试剂盒
    参阅全部 VEGF Receptor 3 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 15 pg/ml
  • 范围
    12.29 pg/ml - 3000 pg/ml
  • 复检

    96 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 97.35 89% - 106%
    Serum 95.46 86% - 104%
    Plasma 96.47 88% - 103%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s VEGF Receptor 3 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VEGF Receptor 3 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human VEGF Receptor 3 coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF Receptor 3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human VEGF Receptor 3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF Receptor 3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性Vascular endothelial growth factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine esidues and sharing common VEGF receptors. VEGFs stimulate the proliferation of endothelial cells, induce angiogenesis, and increase vascular permeability in both large and small vessels. The mitogenic activity of VEGFs appears to be mediated by specific VEGF receptors. VEGF Receptor 3 is one of the five receptor tyrosine kinases (RTKs) (VEGF Receptor 1/Flt1, VEGF Receptor 2/KDR/Flk1, VEGF Receptor 3/Flt4, tie1 and tek/tie2) whose expression is almost exclusively restricted to endothelial cells.
  • 细胞定位Cell membrane; Single-pass type I membrane protein.
  • 别名
    • FLT 4
    • FLT4
    • FLT41
    • Fms like tyrosine kinase 4
    • Fms related tyrosine kinase 4
    • LMPH1A
    • PCL
    • Tyrosine protein kinase receptor FLT4
    • Vascular endothelial growth factor receptor 3
    • VEGFR 3
    • VEGFR3
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human VEGF Receptor 3 ELISA Kit 图像

  • Hu VEGF receptor 3 measured in biofluids showing quantity (pg) per mL of tested sample

  • Representative standard curve using ab100666

  • Representative standard curve using ab100666

实验方案

Human VEGF Receptor 3 ELISA Kit (ab100666)参考文献

ab100666 has not yet been referenced specifically in any publications.

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