人VEGF R1 ELISA试剂盒(FLT1) (ab119613)

概述

  • 产品名称
    人VEGF R1 ELISA试剂盒(FLT1)
    参阅全部 VEGF Receptor 1 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 30 pg/ml
  • 范围
    156 pg/ml - 10000 pg/ml
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Human soluble VEGF R1 (FLT1) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human soluble VEGF R1 (FLT1) in cell culture supernatants, serum and plasma (EDTA).


    A VEGF R1 (FLT1) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for VEGF R1 (FLT1) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution The density of yellow coloration is directly proportional to the Human VEGF R1 (FLT1) amount of sample captured in plate.


    Get higher sensitivity in only 90 minutes with Human VEGF R1 ELISA Kit (FLT1) (ab195210) from our SimpleStep ELISA® range.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab119613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative standard curve using ab119613

实验方案

文献

ab119613 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for your message and I'm sorry to hear you have been having some difficulties.

Optimal dilution of the sample will usually need to be optimized by the end user. In this case, I would appreciate if you could let me know what type of...

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Thanks for your reply.

HRP conjugated secondary antibodies:
1. For ab62183 and ab32152, you can use HRP conjugated secondary antibody ab97051
http://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-HL-HRP-ab97051.html ...

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Thank you for contacting us.

I am happy to confirm that this kitwill detectfulllength and soluble VEGFR1 andtherefore the coating antibody will detect both forms as well.

I hope this information is helpful to you. IPlease do not h...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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