概述

  • 产品名称人VEGF ELISA试剂盒
    参阅全部 VEGFA 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture extracts, Tissue Extracts
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 10 pg/ml
  • 范围
    8.23 pg/ml - 6000 pg/ml
  • 复检

    96 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture extracts 96.79 90% - 107%
    Tissue Extracts 95.58 89% - 106%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s VEGF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VEGF in cell lysates and tissue lysates.

    This assay employs an antibody specific for Human VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human VEGF antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
  • 组织特异性Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
  • 疾病相关Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • 序列相似性Belongs to the PDGF/VEGF growth factor family.
  • 细胞定位Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • Information by UniProt
  • 别名
    • Folliculostellate cell-derived growth factor
    • Glioma-derived endothelial cell mitogen
    • MGC70609
    • MVCD1
    • vascular endothelial growth factor
    • Vascular endothelial growth factor A
    • vascular endothelial growth factor A121
    • vascular endothelial growth factor A165
    • Vascular permeability factor
    • Vegf
    • VEGF A
    • VEGF-A
    • VEGF120
    • Vegfa
    • VEGFA_HUMAN
    • VPF
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human VEGF ELISA Kit 图像

  • Hu VEGF measured in cell lysates showing quantity (pg) per million cells tested

  • Hu VEGF in various fluids showing quantity (pg) per mL of tested sample

  • Standard curve : mean of duplicates (+/-SD) with background readings subtracted

实验方案

Human VEGF ELISA Kit (ab100663)参考文献

This product has been referenced in:
  • Rosser CJ  et al. Urinary protein biomarker panel for the detection of recurrent bladder cancer. Cancer Epidemiol Biomarkers Prev 23:1340-5 (2014). Human . Read more (PubMed: 24714076) »
  • Chang YC  et al. The generation of induced pluripotent stem cells for macular degeneration as a drug screening platform: identification of curcumin as a protective agent for retinal pigment epithelial cells against oxidative stress. Front Aging Neurosci 6:191 (2014). Sandwich ELISA ; Human . Read more (PubMed: 25136316) »

See all 6 Publications for this product

Product Wall


Please note that ab100662 and ab100663 are similar kits that both measure levels of VEGF.

However ab100662 is best used for detecting VEGF in biological fluids such as cell culture supernatant, serum and plasma while ab100663 is best sui...

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Thank you for your enquiry.

Unfortunately, we have been unable to find any references using bone tissue extracts with the VEGF ELISA kit ab100663 nor do we have tips for preparing this type of sample. You may wish to consider trying a furthe...

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Thank you for your enquiry.

I am sorry to confirm we have not tested the effects of Urea containing lysis buffer on samples tested with ab100663 VEGF Human ELISA Kit. We would therefore not be able to confirm how well this would work. Howeve...

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Thank you for contacting us.

The concentration of the HRP-streptavidin reagent is considered proprietary information by the developer of the kit. I am sorry we could not be of more help.

Please do not hesitate to contact us if you...

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I am happy to hear that the new kits are working well for you! Please let me know if there is anything else I can do to help.

Thank you for contacting Abcam regarding our VEGF Human ELISA Kits.


The main difference between ab100663 and ab100662 is the sample types that can be analyzed. Ab100663 can be used to analyze cells and tissues, while ab100662 should be ...

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Thank you for contacting us. We unfortunately are not aware about the purpose of assaying VEGF in cell supernatant or in cell/tissue culture lysates. You may need to search literature to know more about this. The following publication will help to ...

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Thank you for your inquiry and your interest in our products. ab100663 has been specially designed and optimized for cell and tissue lysate samples. If you would like to use different type of sample, I would advise consideraing the serum, plasma, a...

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I sincerely apologize for the mix-up with the kit you had ordered.  I've put through a free of charge replacement of the Item D, 5X sample diluent.  We will ship this out to you once we receive the vial from the supplying lab. Should you still be e...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"