概述

  • 产品名称人VEGF ELISA试剂盒
    参阅全部 VEGFA 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 10 pg/ml
  • 范围
    8.23 pg/ml - 6000 pg/ml
  • 复检

    > 100 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 103.5 92% - 113%
    Serum 104.4 92% - 115%
    Plasma 105.7 93% - 114%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s VEGF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VEGF in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human VEGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
  • 组织特异性Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
  • 疾病相关Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • 序列相似性Belongs to the PDGF/VEGF growth factor family.
  • 细胞定位Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • Information by UniProt
  • 别名
    • MGC70609
    • MVCD1
    • vascular endothelial growth factor
    • Vascular endothelial growth factor A
    • Vascular permeability factor
    • Vegf
    • VEGF A
    • VEGF-A
    • VEGF120
    • Vegfa
    • VEGFA_HUMAN
    • VPF
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100662 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human VEGF ELISA Kit 图像

  • VEGF measured in biological fluids showing quantity (pg) per milliliter of sample tested

  • Curves showing concentrations huVEGF165 in NIBSC 02/286 using ab100662 VEGF human

     

  • Representative standard curve using ab100662

  • Representative standard curve using ab100662

实验方案

Human VEGF ELISA Kit (ab100662)参考文献

This product has been referenced in:
  • Tejero R  et al. miR-141 and miR-200c as markers of overall survival in early stage non-small cell lung cancer adenocarcinoma. PLoS One 9:e101899 (2014). Human . Read more (PubMed: 25003366) »
  • Rocha AL  et al. Activin A stimulates interleukin 8 and vascular endothelial growth factor release from cultured human endometrial stromal cells: possible implications for the pathogenesis of endometriosis. Reprod Sci 19:832-8 (2012). Sandwich ELISA ; Human . Read more (PubMed: 22477338) »

See all 2 Publications for this product

Product Wall



Please find below the information regarding the source/host of our capture and detection antibodies used in our VEGF Human ELISA Kit (ab100662):

Capture Antibody: Monoclonal Mouse IgG2B

Detection Antibody: Polyclonal Goat I...

Read More


Please note that ab100662 and ab100663 are similar kits that both measure levels of VEGF.

However ab100662 is best used for detecting VEGF in biological fluids such as cell culture supernatant, serum and plasma while ab100663 is best sui...

Read More

Unfortunately we no longer test urine as part of the validation. However the kit is very likely to work for urine (based on our past experience with this sample type).

Here are so some general guidelines on preparation of urine for ELISA tes...

Read More



Capture Antibody: Monoclonal, mouse. The immunogen sequence is Ala27­Arg191 (Accession # NP_001165097.1) Detects human VEGF165 and human VEGF121. In ELISAs, this antibody has ˜10% cross­reactivity with recombinant mouse VEGF and rat V...

Read More



The VEGFA isoform that ab100662 detects is 165 and 121.



Answer 1:

Please use 1X Assay Diluent B to dilute the detection antibody and the HRP-Streptavidin. If testing serum/plasma, use Assay Diluent A to prepare/dilute the standards and samples. Where it says use the “appropriate as...

Read More

Thank you for contacting us.

I can confirm that Heparin should not be interfering with the assay using these kits.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or informat...

Read More

Te pido disculpas por la espera.

He recibido una contestación de mis compañeros, que me comentan que la dilución recomendada para suero y plasma debe hacerse entre 2 y 5 veces.

En cualquier caso, los niveles de ...

Read More


Please note this product is unpublished so when you decide to order you need to contact our customer service team for them to put this item in your order.

I hope this info will help. Please let me know if you experience any difficulties.

This product is available but not published on our website. You cannot purchase this using our normal ordering system however I can add this product to your next shipment. Could you confirm how many vials customer would need..6?.
In my last I made...

Read More

1-10 of 14 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"