人VCAM1 ELISA试剂盒(CD106) (ab46118)

概述

  • 产品名称人VCAM1 ELISA试剂盒(CD106)
    参阅全部 VCAM1 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    A 8 48.79ng/ml 0.221 0.45%
    B 8 5.81ng/ml 0.132 2.27%
    批次间
    样品 n Mean SD CV%
    A 28 49.69ng/ml 0.72 1.44%
    B 28 5.95ng/ml 0.354 5.94%
  • 测试
    2 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 0.6 ng/ml
  • 范围
    1.56 ng/ml - 50 ng/ml
  • 检测时间
    1h 40m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Human VCAM1 (CD106) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of VCAM1 in cell culture supernatants, buffered solutions, serum, plasma, and other body fluids.

    A monoclonal antibody specific for VCAM1 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known VCAM1 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for VCAM1 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of VCAM1 present in the samples.

    This kit will recognize both endogenous and recombinant Human VCAM1.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
  • 组织特异性Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
  • 序列相似性Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
  • 结构域Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
  • 翻译后修饰Sialoglycoprotein.
  • 细胞定位Membrane.
  • Information by UniProt
  • 别名
    • CD106
    • CD106 Antigen
    • INCAM 100
    • INCAM-100
    • L1CAM
    • MGC99561
    • V-CAM 1
    • Vascular Cell Adhesion Molecule 1
    • Vascular cell adhesion protein 1
    • VCAM 1
    • VCAM-1
    • VCAM1
    • VCAM1_HUMAN
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab46118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human VCAM1 ELISA Kit (CD106) 图像

  • Representative Standard Curve using ab46118.

实验方案

Human VCAM1 ELISA Kit (CD106) (ab46118)参考文献

ab46118 has not yet been referenced specifically in any publications.

Product Wall

VCAM1 concentration in human plasma

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Human plasma samples were diluted 50 times by diluent buffer.
Username

Abcam user community

Verified customer

提交于 Jun 18 2013

Thank you for contacting us. The laboratory that developed and validated the kit uses linear regression but there are some who argue that 4 parameter logisitic nonlinear regression is a better way to curve-fit ELISA data. Either formula is applicab...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"