人VCAM-1酶联免疫吸附(ELISA)套装(without plates) (ab47355)

概述

  • 产品名称
    人VCAM-1酶联免疫吸附(ELISA)套装(without plates)
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 0.6 ng/ml
  • 范围
    1.56 ng/ml - 50 ng/ml
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s VCAM-1 Human in vitro ELISA Set is designed for the quantitative measurement of VCAM-1 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

     

    This ELISA Set is intended to measure human VCAM-1 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

    The VCAM-1 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

     

    Recognizes both natural and recombinant human VCAM1

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Reagents

性能

  • 相关性
    VCAM1 is important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the integrins alpha4 beta1 (beta 1 integrin VLA4) and alpha4 beta7 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. VCAM1 is also expressed by several non endothelial cell types including some macrophages, follicular dendritic cells and bone marrow, stromal cells.
  • 细胞定位
    Cell Membrane; single-pass type I membrane protein
  • 别名
    • CD106
    • CD106 antigen
    • INCAM 100
    • L1CAM
    • Vascular cell adhesion molecule 1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab47355 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human VCAM-1 ELISA Set (without plates) 图像

  • VCAM-1 Standard curve ranging from 1.56 to 50 ng/mL

Human VCAM-1 ELISA Set (without plates) (ab47355)参考文献

ab47355 has not yet been referenced specifically in any publications.

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Please find below additional info required for ab47355 and ab47361


1) PBS is prepared as followed : 0.137mM NaCl; 1.47 KH2PO4; 8.1mM Na2PO4, 2H2O

2) Samples should be diluted : 1/100 to 1/2...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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